Evolution of IncA/C BlaCMY-₂-carrying Plasmids by Acquisition of the BlaNDM-₁ Carbapenemase Gene

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2011 Nov 30

PMID 22123704

Citations 70

Authors

Alessandra Carattoli

Laura Villa

Laurent Poirel

Remy A Bonnin

Patrice Nordmann

Affiliations

Soon will be listed here.

Abstract

The bla(NDM-1) gene has been reported to be often located on broad-host-range plasmids of the IncA/C type in clinical but also environmental bacteria recovered from the New Delhi, India, area. IncA/C-type plasmids are the main vehicles for the spread of the cephalosporinase gene bla(CMY-2), frequently identified in the United States, Canada, and Europe. In this study, we completed the sequence of IncA/C plasmid pNDM-KN carrying the bla(NDM-1) gene, recovered from a Klebsiella pneumoniae isolate from Kenya. This sequence was compared with those of three IncA/C-type reference plasmids from Escherichia coli, Yersinia ruckeri, and Photobacterium damselae. Comparative analysis showed that the bla(NDM-1) gene was located on a widely diffused plasmid scaffold known to be responsible for the spread of bla(CMY-2)-like genes and consequently for resistance to broad-spectrum cephalosporins. Considering that IncA/C plasmids possess a broad host range, this scaffold might support a large-scale diffusion of the bla(NDM-1) gene among Gram-negative rods.

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References

Poirel L, Revathi G, Bernabeu S, Nordmann P . Detection of NDM-1-producing Klebsiella pneumoniae in Kenya. Antimicrob Agents Chemother. 2010; 55(2):934-6. PMC: 3028766. DOI: 10.1128/AAC.01247-10. View

Poirel L, Dortet L, Bernabeu S, Nordmann P . Genetic features of blaNDM-1-positive Enterobacteriaceae. Antimicrob Agents Chemother. 2011; 55(11):5403-7. PMC: 3195013. DOI: 10.1128/AAC.00585-11. View

Poole T, Edrington T, Brichta-Harhay D, Carattoli A, Anderson R, Nisbet D . Conjugative transferability of the A/C plasmids from Salmonella enterica isolates that possess or lack bla(CMY) in the A/C plasmid backbone. Foodborne Pathog Dis. 2009; 6(10):1185-94. DOI: 10.1089/fpd.2009.0316. View

Hopkins K, Liebana E, Villa L, Batchelor M, Threlfall E, Carattoli A . Replicon typing of plasmids carrying CTX-M or CMY beta-lactamases circulating among Salmonella and Escherichia coli isolates. Antimicrob Agents Chemother. 2006; 50(9):3203-6. PMC: 1563510. DOI: 10.1128/AAC.00149-06. View

Walsh T, Weeks J, Livermore D, Toleman M . Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study. Lancet Infect Dis. 2011; 11(5):355-62. DOI: 10.1016/S1473-3099(11)70059-7. View

Carattoli A, Miriagou V, Bertini A, Loli A, Colinon C, Villa L . Replicon typing of plasmids encoding resistance to newer beta-lactams. Emerg Infect Dis. 2006; 12(7):1145-8. PMC: 3291055. DOI: 10.3201/eid1207.051555. View

Poirel L, Decousser J, Nordmann P . Insertion sequence ISEcp1B is involved in expression and mobilization of a bla(CTX-M) beta-lactamase gene. Antimicrob Agents Chemother. 2003; 47(9):2938-45. PMC: 182628. DOI: 10.1128/AAC.47.9.2938-2945.2003. View

Poirel L, Bonnin R, Nordmann P . Analysis of the resistome of a multidrug-resistant NDM-1-producing Escherichia coli strain by high-throughput genome sequencing. Antimicrob Agents Chemother. 2011; 55(9):4224-9. PMC: 3165296. DOI: 10.1128/AAC.00165-11. View

Kaase M, Nordmann P, Wichelhaus T, Gatermann S, Bonnin R, Poirel L . NDM-2 carbapenemase in Acinetobacter baumannii from Egypt. J Antimicrob Chemother. 2011; 66(6):1260-2. DOI: 10.1093/jac/dkr135. View

10.

Mataseje L, Baudry P, Zhanel G, Morck D, Read R, Louie M . Comparison of CMY-2 plasmids isolated from human, animal, and environmental Escherichia coli and Salmonella spp. from Canada. Diagn Microbiol Infect Dis. 2010; 67(4):387-91. DOI: 10.1016/j.diagmicrobio.2010.02.027. View

11.

Colinon C, Miriagou V, Carattoli A, Luzzaro F, Rossolini G . Characterization of the IncA/C plasmid pCC416 encoding VIM-4 and CMY-4 beta-lactamases. J Antimicrob Chemother. 2007; 60(2):258-62. DOI: 10.1093/jac/dkm171. View

12.

Sidjabat H, Nimmo G, Walsh T, Binotto E, Htin A, Hayashi Y . Carbapenem resistance in Klebsiella pneumoniae due to the New Delhi Metallo-β-lactamase. Clin Infect Dis. 2011; 52(4):481-4. PMC: 3106237. DOI: 10.1093/cid/ciq178. View

13.

Carattoli A, Tosini F, Giles W, Rupp M, Hinrichs S, Angulo F . Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella strains isolated in the United States between 1996 and 1998. Antimicrob Agents Chemother. 2002; 46(5):1269-72. PMC: 127137. DOI: 10.1128/AAC.46.5.1269-1272.2002. View

14.

Lindsey R, Fedorka-Cray P, Frye J, Meinersmann R . Inc A/C plasmids are prevalent in multidrug-resistant Salmonella enterica isolates. Appl Environ Microbiol. 2009; 75(7):1908-15. PMC: 2663206. DOI: 10.1128/AEM.02228-08. View

15.

Yong D, Toleman M, Giske C, Cho H, Sundman K, Lee K . Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother. 2009; 53(12):5046-54. PMC: 2786356. DOI: 10.1128/AAC.00774-09. View

16.

Karthikeyan K, Thirunarayan M, Krishnan P . Coexistence of blaOXA-23 with blaNDM-1 and armA in clinical isolates of Acinetobacter baumannii from India. J Antimicrob Chemother. 2010; 65(10):2253-4. DOI: 10.1093/jac/dkq273. View

17.

Kumarasamy K, Toleman M, Walsh T, Bagaria J, Butt F, Balakrishnan R . Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. Lancet Infect Dis. 2010; 10(9):597-602. PMC: 2933358. DOI: 10.1016/S1473-3099(10)70143-2. View

18.

Daniels J, Call D, Besser T . Molecular epidemiology of blaCMY-2 plasmids carried by Salmonella enterica and Escherichia coli isolates from cattle in the Pacific Northwest. Appl Environ Microbiol. 2007; 73(24):8005-11. PMC: 2168131. DOI: 10.1128/AEM.01325-07. View

19.

Johnson T, Wannemuehler Y, Johnson S, Logue C, White D, Doetkott C . Plasmid replicon typing of commensal and pathogenic Escherichia coli isolates. Appl Environ Microbiol. 2007; 73(6):1976-83. PMC: 1828809. DOI: 10.1128/AEM.02171-06. View

20.

Poirel L, Villa L, Bertini A, Pitout J, Nordmann P, Carattoli A . Expanded-spectrum beta-lactamase and plasmid-mediated quinolone resistance. Emerg Infect Dis. 2007; 13(5):803-5. PMC: 2738457. DOI: 10.3201/eid1305.061293. View