Genome-wide Functional Screening of MiR-23b As a Pleiotropic Modulator Suppressing Cancer Metastasis

Overview

Journal Nat Commun

Specialty Biology

Date 2011 Nov 24

PMID 22109528

Citations 109

Authors

Hanshuo Zhang

Yang Hao

Junyu Yang

Ying Zhou

Juan Li

Shenyi Yin

Changhong Sun

Ming Ma

Yanyi Huang

Jianzhong Jeff Xi

Affiliations

Soon will be listed here.

Abstract

miRNA globally deregulates human carcinoma. A critical open question is how many miRNAs functionally participate in cancer development, particularly in metastasis. We systematically evaluate the capability of all known human miRNAs to regulate certain metastasis-relevant cell behaviours. To perform the high-throughput screen of miRNAs, which regulate cell migration, we developed a novel self-assembled cell microarray. Here we show that over 20% of miRNAs have migratory regulation activity in diverse cell types, indicating a general involvement of miRNAs in migratory regulation. MiR-23b, which is downregulated in human colon cancer samples, potently mediates the multiple steps of metastasis, including tumour growth, invasion and angiogenesis in vivo. It regulates a cohort of prometastatic targets, including FZD7 or MAP3k1. These findings provide new insight into the physiological and potential therapeutic importance of miRNAs as a new class of functional modulators.

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