Strengthening Relationships: Amyloids Create Adhesion Nanodomains in Yeasts

Overview

Journal Trends Microbiol

Date 2011 Nov 22

PMID 22099004

Citations 58

Authors

Peter N Lipke

Melissa C Garcia

David Alsteens

Caleen B Ramsook

Stephen A Klotz

Yves F Dufrene

Affiliations

Soon will be listed here.

Abstract

Budding yeasts adhere to biotic or abiotic surfaces and aggregate to form biofilms, using wall-anchored glycoprotein adhesins. The process is paradoxical: adhesins often show weak binding to specific ligands, yet mediate remarkably strong adherence. Single-molecule atomic force microscopy (AFM), genomics, biochemistry and cell biology have recently explained the puzzle, with Candida albicans Als adhesins as the paradigm. The strength of adhesion results partly from force-activated amyloid-like clustering of hundreds of adhesin molecules to form arrays of ordered multimeric binding sites. The various protein domains of eukaryotic adhesins cooperate to facilitate this fascinating new mechanism of activation.

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