The Xenopus Laevis Poly(A) Binding Protein is Composed of Multiple Functionally Independent RNA Binding Domains

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1990 Nov 1

PMID 2209558

Citations 64

Authors

W Nietfeld

H Mentzel

T Pieler

Affiliations

Soon will be listed here.

Abstract

A family of eukaryotic RNA binding proteins is defined by the conserved RNP motif. The poly(A) binding protein has four such motifs. We report on the isolation and structural characterization of several variant cDNA clones, as well as of a gene encoding this protein in Xenopus laevis embryos. Wild-type protein as well as truncated versions carrying isolated single motifs or artificial combinations of two and more such elements were characterized for their ability to bind specifically to RNA homopolymers. Three of the isolated repeats were functional in specific RNA binding, whereas the N-terminal RNP motif was non-functional. Combinatorial effects in RNA binding were measured with constructs carrying multiple repeats, which were not predictable from the activity of isolated domains.

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References

Bourbon H, Lapeyre B, Amalric F . Structure of the mouse nucleolin gene. The complete sequence reveals that each RNA binding domain is encoded by two independent exons. J Mol Biol. 1988; 200(4):627-38. DOI: 10.1016/0022-2836(88)90476-7. View

Swanson M, Dreyfuss G . Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities. Mol Cell Biol. 1988; 8(5):2237-41. PMC: 363409. DOI: 10.1128/mcb.8.5.2237-2241.1988. View

Bell L, Maine E, Schedl P, Cline T . Sex-lethal, a Drosophila sex determination switch gene, exhibits sex-specific RNA splicing and sequence similarity to RNA binding proteins. Cell. 1988; 55(6):1037-46. DOI: 10.1016/0092-8674(88)90248-6. View

Query C, Bentley R, Keene J . A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein. Cell. 1989; 57(1):89-101. DOI: 10.1016/0092-8674(89)90175-x. View

Dreyfuss G, Swanson M, Pinol-Roma S . Heterogeneous nuclear ribonucleoprotein particles and the pathway of mRNA formation. Trends Biochem Sci. 1988; 13(3):86-91. DOI: 10.1016/0968-0004(88)90046-1. View

Bandziulis R, Swanson M, Dreyfuss G . RNA-binding proteins as developmental regulators. Genes Dev. 1989; 3(4):431-7. DOI: 10.1101/gad.3.4.431. View

Zelus B, Giebelhaus D, Eib D, Kenner K, Moon R . Expression of the poly(A)-binding protein during development of Xenopus laevis. Mol Cell Biol. 1989; 9(6):2756-60. PMC: 362352. DOI: 10.1128/mcb.9.6.2756-2760.1989. View

Sachs A, Davis R . The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation. Cell. 1989; 58(5):857-67. DOI: 10.1016/0092-8674(89)90938-0. View

Nietfeld W, Mentzel H, Pieler T, Koster M, Poting A, Knochel W . Second-order repeats in Xenopus laevis finger proteins. J Mol Biol. 1989; 208(4):639-59. DOI: 10.1016/0022-2836(89)90155-1. View

10.

Scherly D, Boelens W, van Venrooij W, Dathan N, Hamm J, Mattaj I . Identification of the RNA binding segment of human U1 A protein and definition of its binding site on U1 snRNA. EMBO J. 1989; 8(13):4163-70. PMC: 401606. DOI: 10.1002/j.1460-2075.1989.tb08601.x. View

11.

Fan C, Maniatis T . A DNA-binding protein containing two widely separated zinc finger motifs that recognize the same DNA sequence. Genes Dev. 1990; 4(1):29-42. DOI: 10.1101/gad.4.1.29. View

12.

Rackwitz H, Zehetner G, Frischauf A, Lehrach H . Rapid restriction mapping of DNA cloned in lambda phage vectors. Gene. 1984; 30(1-3):195-200. DOI: 10.1016/0378-1119(84)90120-3. View

13.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

14.

Scherly D, Boelens W, Dathan N, van Venrooij W, Mattaj I . Major determinants of the specificity of interaction between small nuclear ribonucleoproteins U1A and U2B'' and their cognate RNAs. Nature. 1990; 345(6275):502-6. DOI: 10.1038/345502a0. View

15.

Krieg P, Melton D . Developmental regulation of a gastrula-specific gene injected into fertilized Xenopus eggs. EMBO J. 1985; 4(13A):3463-71. PMC: 554685. DOI: 10.1002/j.1460-2075.1985.tb04105.x. View

16.

Cobianchi F, SENGUPTA D, Zmudzka B, Wilson S . Structure of rodent helix-destabilizing protein revealed by cDNA cloning. J Biol Chem. 1986; 261(8):3536-43. View

17.

Sachs A, Bond M, Kornberg R . A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression. Cell. 1986; 45(6):827-35. DOI: 10.1016/0092-8674(86)90557-x. View

18.

Lapeyre B, Bourbon H, Amalric F . Nucleolin, the major nucleolar protein of growing eukaryotic cells: an unusual protein structure revealed by the nucleotide sequence. Proc Natl Acad Sci U S A. 1987; 84(6):1472-6. PMC: 304456. DOI: 10.1073/pnas.84.6.1472. View

19.

Grange T, De Sa C, Oddos J, Pictet R . Human mRNA polyadenylate binding protein: evolutionary conservation of a nucleic acid binding motif. Nucleic Acids Res. 1987; 15(12):4771-87. PMC: 305917. DOI: 10.1093/nar/15.12.4771. View

20.

Sachs A, Davis R, Kornberg R . A single domain of yeast poly(A)-binding protein is necessary and sufficient for RNA binding and cell viability. Mol Cell Biol. 1987; 7(9):3268-76. PMC: 367964. DOI: 10.1128/mcb.7.9.3268-3276.1987. View