Rapid Restriction Mapping of DNA Cloned in Lambda Phage Vectors

Overview

Journal Gene

Specialty Molecular Biology

Date 1984 Oct 1

PMID 6096214

Citations 91

Authors

H R Rackwitz

G Zehetner

A M Frischauf

H Lehrach

Affiliations

Soon will be listed here.

Abstract

A protocol for the rapid restriction mapping of phage lambda clones has been developed. Partial digestion products are selectively labelled at the right or left cohesive lambda DNA termini by hybridisation with [32P]oligonucleotides complementary to the single-stranded cos ends. After gel electrophoresis and autoradiography, the restriction map can be directly determined from the "ladder" of partial digestion products.

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