Identification, Purification, and Biological Characterization of Hematopoietic Stem Cell Factor from Buffalo Rat Liver--conditioned Medium

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 1990 Oct 5

PMID 2208278

Citations 130

Authors

K M Zsebo

J Wypych

I K McNiece

H S Lu

K A Smith

S B Karkare

R K Sachdev

V N Yuschenkoff

N C Birkett

L R Williams

Affiliations

Soon will be listed here.

Abstract

We have identified a novel growth factor, stem cell factor (SCF), for primitive hematopoietic progenitors based on its activity on bone marrow cells derived from mice treated with 5-fluorouracil. The protein was isolated from the medium conditioned by Buffalo rat liver cells. It is heavily glycosylated, with both N-linked and O-linked carbohydrate. Amino acid sequence following removal of N-terminal pyroglutamate is presented. The protein has potent synergistic activities in semisolid bone marrow cultures in conjunction with colony-stimulating factors. It is also a growth factor for mast cells. In two companion papers, we present the sequences of partial SCF cDNAs, identify SCF as a c-kit ligand, and map the SCF gene to the Sl locus of the mouse.

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