Identification, Sequencing and Expression of an Integral Membrane Protein of the Trans-Golgi Network (TGN38)

Overview

Journal Biochem J

Specialty Biochemistry

Date 1990 Aug 15

PMID 2204342

Citations 146

Authors

J P Luzio

B Brake

G Banting

K E Howell

P Braghetta

K K Stanley

Affiliations

Soon will be listed here.

Abstract

Organelle-specific integral membrane proteins were identified by a novel strategy which gives rise to monospecific antibodies to these proteins as well as to the cDNA clones encoding them. A cDNA expression library was screened with a polyclonal antiserum raised against Triton X-114-extracted organelle proteins and clones were then grouped using antibodies affinity-purified on individual fusion proteins. The identification, molecular cloning and sequencing are described of a type 1 membrane protein (TGN38) which is located specifically in the trans-Golgi network.

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