Human Breast Cancer Histoid: an in Vitro 3-dimensional Co-culture Model That Mimics Breast Cancer Tissue

Overview

Journal J Histochem Cytochem

Publisher Sage Publications

Specialty Biochemistry

Date 2011 Oct 29

PMID 22034518

Citations 21

Authors

Pavinder Kaur

Brenda Ward

Baisakhi Saha

Lillian Young

Susan Groshen

Geza Techy

Yani Lu

Roscoe Atkinson

Clive R Taylor

Marylou Ingram

S Ashraf Imam

Affiliations

Soon will be listed here.

Abstract

Progress in our understanding of heterotypic cellular interaction in the tumor microenvironment, which is recognized to play major roles in cancer progression, has been hampered due to unavailability of an appropriate in vitro co-culture model. The aim of this study was to generate an in vitro 3-dimensional human breast cancer model, which consists of cancer cells and fibroblasts. Breast cancer cells (UACC-893) and fibroblasts at various densities were co-cultured in a rotating suspension culture system to establish co-culture parameters. Subsequently, UACC-893, BT.20, or MDA.MB.453 were co-cultured with fibroblasts for 9 days. Co-cultures resulted in the generation of breast cancer histoid (BCH) with cancer cells showing the invasion of fibroblast spheroids, which were visualized by immunohistochemical (IHC) staining of sections (4 µm thick) of BCH. A reproducible quantitative expression of C-erbB.2 was detected in UACC-893 cancer cells in BCH sections by IHC staining and the Automated Cellular Imaging System. BCH sections also consistently exhibited qualitative expression of pancytokeratins, p53, Ki-67, or E-cadherin in cancer cells and that of vimentin or GSTPi in fibroblasts, fibronectin in the basement membrane and collagen IV in the extracellular matrix. The expression of the protein analytes and cellular architecture of BCH were markedly similar to those of breast cancer tissue.

Citing Articles

Mesenchymal stem cell origin contributes to the antitumor effect of oncolytic virus carriers.

Sukegawa M, Miyagawa Y, Kuroda S, Yamazaki Y, Yamamoto M, Adachi K Mol Ther Oncol. 2024; 32(4):200896.

PMID: 39554905 PMC: 11568361. DOI: 10.1016/j.omton.2024.200896.

Three Dimensional Models of Endocrine Organs and Target Tissues Regulated by the Endocrine System.

Luca E, Zitzmann K, Bornstein S, Kugelmeier P, Beuschlein F, Nolting S Cancers (Basel). 2023; 15(18).

PMID: 37760571 PMC: 10526768. DOI: 10.3390/cancers15184601.

The role of three-dimensional in vitro models in modelling the inflammatory microenvironment associated with obesity in breast cancer.

Blyth R, Birts C, Beers S Breast Cancer Res. 2023; 25(1):104.

PMID: 37697381 PMC: 10494415. DOI: 10.1186/s13058-023-01700-w.

The Variety of 3D Breast Cancer Models for the Study of Tumor Physiology and Drug Screening.

Frohlich E Int J Mol Sci. 2023; 24(8).

PMID: 37108283 PMC: 10139112. DOI: 10.3390/ijms24087116.

Cancer Spheroids and Organoids as Novel Tools for Research and Therapy: State of the Art and Challenges to Guide Precision Medicine.

El Harane S, Zidi B, El Harane N, Krause K, Matthes T, Preynat-Seauve O Cells. 2023; 12(7).

PMID: 37048073 PMC: 10093533. DOI: 10.3390/cells12071001.

References

Press M, Sauter G, Bernstein L, Villalobos I, Mirlacher M, Zhou J . Diagnostic evaluation of HER-2 as a molecular target: an assessment of accuracy and reproducibility of laboratory testing in large, prospective, randomized clinical trials. Clin Cancer Res. 2005; 11(18):6598-607. DOI: 10.1158/1078-0432.CCR-05-0636. View

Gallagher P, Bao Y, Prorock A, Zigrino P, Nischt R, Politi V . Gene expression profiling reveals cross-talk between melanoma and fibroblasts: implications for host-tumor interactions in metastasis. Cancer Res. 2005; 65(10):4134-46. DOI: 10.1158/0008-5472.CAN-04-0415. View

Minot D, Kipp B, Root R, Meyer R, Reynolds C, Nassar A . Automated cellular imaging system III for assessing HER2 status in breast cancer specimens: development of a standardized scoring method that correlates with FISH. Am J Clin Pathol. 2009; 132(1):133-8. DOI: 10.1309/AJCPJV0SKAF2PCMY. View

Gudjonsson T, Ronnov-Jessen L, Villadsen R, Bissell M, Petersen O . To create the correct microenvironment: three-dimensional heterotypic collagen assays for human breast epithelial morphogenesis and neoplasia. Methods. 2003; 30(3):247-55. PMC: 2933212. DOI: 10.1016/s1046-2023(03)00031-8. View

Selvey S, Haupt L, Thompson E, Matthaei K, Irving M, Griffiths L . Stimulation of MMP-11 (stromelysin-3) expression in mouse fibroblasts by cytokines, collagen and co-culture with human breast cancer cell lines. BMC Cancer. 2004; 4:40. PMC: 503389. DOI: 10.1186/1471-2407-4-40. View

Desmouliere A, Guyot C, Gabbiani G . The stroma reaction myofibroblast: a key player in the control of tumor cell behavior. Int J Dev Biol. 2004; 48(5-6):509-17. DOI: 10.1387/ijdb.041802ad. View

Nakagawa H, Liyanarachchi S, Davuluri R, Auer H, Martin Jr E, de la Chapelle A . Role of cancer-associated stromal fibroblasts in metastatic colon cancer to the liver and their expression profiles. Oncogene. 2004; 23(44):7366-77. DOI: 10.1038/sj.onc.1208013. View

Sung S, Hsieh C, Law A, Zhau H, Pathak S, Multani A . Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis. Cancer Res. 2008; 68(23):9996-10003. PMC: 3105756. DOI: 10.1158/0008-5472.CAN-08-2492. View

Che Z, Jung T, Choi J, Yoon D, Jeong H, Lee E . Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction. Biochem Biophys Res Commun. 2006; 346(1):268-75. DOI: 10.1016/j.bbrc.2006.05.111. View

10.

Kenny P, Lee G, Myers C, Neve R, Semeiks J, Spellman P . The morphologies of breast cancer cell lines in three-dimensional assays correlate with their profiles of gene expression. Mol Oncol. 2008; 1(1):84-96. PMC: 2391005. DOI: 10.1016/j.molonc.2007.02.004. View

11.

Sung K, Yang N, Pehlke C, Keely P, Eliceiri K, Friedl A . Transition to invasion in breast cancer: a microfluidic in vitro model enables examination of spatial and temporal effects. Integr Biol (Camb). 2010; 3(4):439-50. PMC: 3094750. DOI: 10.1039/c0ib00063a. View

12.

Gudjonsson T, Ronnov-Jessen L, Villadsen R, Rank F, Bissell M, Petersen O . Normal and tumor-derived myoepithelial cells differ in their ability to interact with luminal breast epithelial cells for polarity and basement membrane deposition. J Cell Sci. 2002; 115(Pt 1):39-50. PMC: 2933194. DOI: 10.1242/jcs.115.1.39. View

13.

Rhee H, Zhau H, Pathak S, Multani A, Pennanen S, Visakorpi T . Permanent phenotypic and genotypic changes of prostate cancer cells cultured in a three-dimensional rotating-wall vessel. In Vitro Cell Dev Biol Anim. 2001; 37(3):127-40. DOI: 10.1290/1071-2690(2001)037<0127:PPAGCO>2.0.CO;2. View

14.

Hsiao A, Torisawa Y, Tung Y, Sud S, Taichman R, Pienta K . Microfluidic system for formation of PC-3 prostate cancer co-culture spheroids. Biomaterials. 2009; 30(16):3020-7. PMC: 2675053. DOI: 10.1016/j.biomaterials.2009.02.047. View

15.

Han J, Chang H, Giricz O, Lee G, Baehner F, Gray J . Molecular predictors of 3D morphogenesis by breast cancer cell lines in 3D culture. PLoS Comput Biol. 2010; 6(2):e1000684. PMC: 2829039. DOI: 10.1371/journal.pcbi.1000684. View

16.

Slamon D, Clark G, Wong S, Levin W, Ullrich A, McGuire W . Human breast cancer: correlation of relapse and survival with amplification of the HER-2/neu oncogene. Science. 1987; 235(4785):177-82. DOI: 10.1126/science.3798106. View

17.

Patocs A, Zhang L, Xu Y, Weber F, Caldes T, Mutter G . Breast-cancer stromal cells with TP53 mutations and nodal metastases. N Engl J Med. 2007; 357(25):2543-51. DOI: 10.1056/NEJMoa071825. View

18.

Galie M, Sorrentino C, Montani M, Micossi L, Di Carlo E, DAntuono T . Mammary carcinoma provides highly tumourigenic and invasive reactive stromal cells. Carcinogenesis. 2005; 26(11):1868-78. DOI: 10.1093/carcin/bgi158. View

19.

Ingram M, Techy G, Ward B, Imam S, Atkinson R, Ho H . Tissue engineered tumor models. Biotech Histochem. 2010; 85(4):213-29. DOI: 10.3109/10520295.2010.483655. View

20.

Micke P, Ostman A . Exploring the tumour environment: cancer-associated fibroblasts as targets in cancer therapy. Expert Opin Ther Targets. 2005; 9(6):1217-33. DOI: 10.1517/14728222.9.6.1217. View