Novel Flaviviruses Detected in Different Species of Mosquitoes in Spain

Overview

Journal Vector Borne Zoonotic Dis

Specialties Infectious Diseases
Public Health
Veterinary Medicine

Date 2011 Oct 26

PMID 22022811

Citations 65

Authors

Ana Vazquez

Maria-Paz Sanchez-Seco

Gustavo Palacios

Francisca Molero

Noelia Reyes

Santiago Ruiz

Carles Aranda

Eduard Marques

Raul Escosa

Juana Moreno

Jordi Figuerola

Antonio Tenorio

Affiliations

Soon will be listed here.

Abstract

We report the characterization of three novel flaviviruses isolated in Spain. Marisma Mosquito virus, a novel mosquito borne virus, was isolated from Ochlerotatus caspius mosquitoes; Spanish Ochlerotatus flavivirus and Spanish Culex flavivirus, two novel insect flaviviruses, were isolated from Oc. caspius and Culex pipiens, respectively. During this investigation, we designed a sensitive RT-nested polymerase chain reaction method that amplifies a 1019bp fragment of the flavivirus NS5 gene and could be directly used in clinical or environmental samples for flavivirus characterization and surveillance. Analysis of the sequence generated from that amplicon contains enough phylogenetic information for proper taxonomic studies. Moreover, the use of this tool allowed the detection of additional flavivirus DNA forms in Culex, Culiseta, and Ochlerotatus mosquitoes.

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References

Morales-Betoulle M, Monzon Pineda M, Sosa S, Panella N, Lopez M, Cordon-Rosales C . Culex flavivirus isolates from mosquitoes in Guatemala. J Med Entomol. 2008; 45(6):1187-90. DOI: 10.1603/0022-2585(2008)45[1187:cfifmi]2.0.co;2. View

Junglen S, Kopp A, Kurth A, Pauli G, Ellerbrok H, Leendertz F . A new flavivirus and a new vector: characterization of a novel flavivirus isolated from uranotaenia mosquitoes from a tropical rain forest. J Virol. 2009; 83(9):4462-8. PMC: 2668441. DOI: 10.1128/JVI.00014-09. View

Stollar V, Thomas V . An agent in the Aedes aegypti cell line (Peleg) which causes fusion of Aedes albopictus cells. Virology. 1975; 64(2):367-77. DOI: 10.1016/0042-6822(75)90113-0. View

Sanchez-Seco M, Vazquez A, Collao X, Hernandez L, Aranda C, Ruiz S . Surveillance of arboviruses in Spanish wetlands: detection of new flavi- and phleboviruses. Vector Borne Zoonotic Dis. 2009; 10(2):203-6. DOI: 10.1089/vbz.2008.0188. View

Farfan-Ale J, Lorono-Pino M, Garcia-Rejon J, Hovav E, Powers A, Lin M . Detection of RNA from a novel West Nile-like virus and high prevalence of an insect-specific flavivirus in mosquitoes in the Yucatan Peninsula of Mexico. Am J Trop Med Hyg. 2009; 80(1):85-95. PMC: 2663380. View

Posada D . Using MODELTEST and PAUP* to select a model of nucleotide substitution. Curr Protoc Bioinformatics. 2008; Chapter 6:Unit 6.5. DOI: 10.1002/0471250953.bi0605s00. View

Kihara Y, Satho T, Eshita Y, Sakai K, Kotaki A, Takasaki T . Rapid determination of viral RNA sequences in mosquitoes collected in the field. J Virol Methods. 2007; 146(1-2):372-4. DOI: 10.1016/j.jviromet.2007.07.008. View

Hoshino K, Isawa H, Tsuda Y, Sawabe K, Kobayashi M . Isolation and characterization of a new insect flavivirus from Aedes albopictus and Aedes flavopictus mosquitoes in Japan. Virology. 2009; 391(1):119-29. DOI: 10.1016/j.virol.2009.06.025. View

Vazquez A, Sanchez-Seco M, Ruiz S, Molero F, Hernandez L, Moreno J . Putative new lineage of west nile virus, Spain. Emerg Infect Dis. 2010; 16(3):549-52. PMC: 3322021. DOI: 10.3201/eid1603.091033. View

10.

Huhtamo E, Putkuri N, Kurkela S, Manni T, Vaheri A, Vapalahti O . Characterization of a novel flavivirus from mosquitoes in northern europe that is related to mosquito-borne flaviviruses of the tropics. J Virol. 2009; 83(18):9532-40. PMC: 2738272. DOI: 10.1128/JVI.00529-09. View

11.

Crochu S, Cook S, Attoui H, Charrel R, de Chesse R, Belhouchet M . Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. mosquitoes. J Gen Virol. 2004; 85(Pt 7):1971-1980. DOI: 10.1099/vir.0.79850-0. View

12.

Kanthong N, Khemnu N, Pattanakitsakul S, Malasit P, Flegel T . Persistent, triple-virus co-infections in mosquito cells. BMC Microbiol. 2010; 10:14. PMC: 2817673. DOI: 10.1186/1471-2180-10-14. View

13.

Cook S, Bennett S, Holmes E, de Chesse R, Moureau G, de Lamballerie X . Isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from Puerto Rico. J Gen Virol. 2006; 87(Pt 4):735-748. DOI: 10.1099/vir.0.81475-0. View

14.

Pabbaraju K, Ho K, Wong S, Fox J, Kaplen B, Tyler S . Surveillance of mosquito-borne viruses in Alberta using reverse transcription polymerase chain reaction with generic primers. J Med Entomol. 2009; 46(3):640-8. DOI: 10.1603/033.046.0332. View

15.

Roiz D, Vazquez A, Sanchez Seco M, Tenorio A, Rizzoli A . Detection of novel insect flavivirus sequences integrated in Aedes albopictus (Diptera: Culicidae) in Northern Italy. Virol J. 2009; 6:93. PMC: 2714537. DOI: 10.1186/1743-422X-6-93. View

16.

Calisher C, Gould E . Taxonomy of the virus family Flaviviridae. Adv Virus Res. 2003; 59:1-19. DOI: 10.1016/s0065-3527(03)59001-7. View

17.

ROZEBOOM L, Kassira E . Dual infections of mosquitoes with strains of West Nile virus. J Med Entomol. 1969; 6(4):407-11. DOI: 10.1093/jmedent/6.4.407. View

18.

Cook S, Moureau G, Harbach R, Mukwaya L, Goodger K, Ssenfuka F . Isolation of a novel species of flavivirus and a new strain of Culex flavivirus (Flaviviridae) from a natural mosquito population in Uganda. J Gen Virol. 2009; 90(Pt 11):2669-2678. PMC: 2885038. DOI: 10.1099/vir.0.014183-0. View

19.

Kim D, Guzman H, Bueno Jr R, Dennett J, Auguste A, Carrington C . Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad. Virology. 2009; 386(1):154-9. DOI: 10.1016/j.virol.2008.12.034. View

20.

Vazquez A, Ruiz S, Herrero L, Moreno J, Molero F, Magallanes A . West Nile and Usutu viruses in mosquitoes in Spain, 2008-2009. Am J Trop Med Hyg. 2011; 85(1):178-81. PMC: 3122364. DOI: 10.4269/ajtmh.2011.11-0042. View