A New Flavivirus and a New Vector: Characterization of a Novel Flavivirus Isolated from Uranotaenia Mosquitoes from a Tropical Rain Forest

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2009 Feb 20

PMID 19224998

Citations 69

Authors

Sandra Junglen

Anne Kopp

Andreas Kurth

Georg Pauli

Heinz Ellerbrok

Fabian H Leendertz

Affiliations

Soon will be listed here.

Abstract

A novel flavivirus was isolated from Uranotaenia mashonaensis, a mosquito genus not previously known to harbor flaviviruses. Mosquitoes were caught in the primary rain forest of the Taï National Park, Côte d'Ivoire. The novel virus, termed nounané virus (NOUV), seemed to grow only on C6/36 insect cells and not on vertebrate cells. Typical enveloped flavivirus-like particles of 60 to 65 nm in diameter were detected by electron microscopy in the cell culture supernatant of infected cells. The full genome was sequenced, and potential cleavage and glycosylation sites and cysteine residues were identified, suggesting that the processing of the NOUV polyprotein is similar to that of other flaviviruses. Phylogenetic analyses of the whole polyprotein and the NS3 protein showed that the virus forms a distinct cluster within the clade of mosquito-borne flaviviruses. Only a distant relationship to other known flaviviruses was found, indicating that NOUV is a novel lineage within the Flaviviridae.

Citing Articles

hsa-miR-548d-3p: a potential microRNA to target nucleocapsid and/or capsid genes in multiple members of the Flaviviridae family.

Cayatineto H, Hakim S Front Bioinform. 2025; 4:1487292.

PMID: 39877236 PMC: 11772435. DOI: 10.3389/fbinf.2024.1487292.

Genotypic and phylogeographic insights into a pre-epidemic variant of Wesselsbron virus detected in sylvatic from Semuliki Forest, Uganda.

Eibner G, Graff S, Hieke C, Ochieng J, Kopp A, Drosten C Microbiol Spectr. 2024; :e0091424.

PMID: 39530699 PMC: 11619370. DOI: 10.1128/spectrum.00914-24.

Pan-flavivirus analysis reveals sfRNA-independent, 3' UTR-biased siRNA production from an insect-specific flavivirus.

Besson B, Overheul G, Wolfinger M, Junglen S, van Rij R J Virol. 2024; 98(11):e0121524.

PMID: 39404457 PMC: 11575252. DOI: 10.1128/jvi.01215-24.

Orthoflavivirus Lammi in Russia: Possible Transovarial Transmission and Trans-Stadial Survival in (Diptera, Culicidae).

Kholodilov I, Aibulatov S, Khalin A, Polienko A, Klimentov A, Belova O Viruses. 2024; 16(4).

PMID: 38675870 PMC: 11054007. DOI: 10.3390/v16040527.

Viral diversity and blood-feeding patterns of Afrotropical biting midges (Diptera: Ceratopogonidae).

Ogola E, Bastos A, Slothouwer I, Getugi C, Osalla J, Omoga D Front Microbiol. 2024; 14:1325473.

PMID: 38249470 PMC: 10797016. DOI: 10.3389/fmicb.2023.1325473.

References

Stang A, Korn K, Wildner O, Uberla K . Characterization of virus isolates by particle-associated nucleic acid PCR. J Clin Microbiol. 2005; 43(2):716-20. PMC: 548055. DOI: 10.1128/JCM.43.2.716-720.2005. View

Monath T . Yellow fever: an update. Lancet Infect Dis. 2002; 1(1):11-20. DOI: 10.1016/S1473-3099(01)00016-0. View

Nowak T, WENGLER G . Analysis of disulfides present in the membrane proteins of the West Nile flavivirus. Virology. 1987; 156(1):127-37. DOI: 10.1016/0042-6822(87)90443-0. View

Nisbet D, Lee K, van den Hurk A, Johansen C, Kuno G, Chang G . Identification of new flaviviruses in the Kokobera virus complex. J Gen Virol. 2004; 86(Pt 1):121-124. DOI: 10.1099/vir.0.80381-0. View

Mandl C, Heinz F, STOCKL E, Kunz C . Genome sequence of tick-borne encephalitis virus (Western subtype) and comparative analysis of nonstructural proteins with other flaviviruses. Virology. 1989; 173(1):291-301. DOI: 10.1016/0042-6822(89)90246-8. View

Kuno G, Chang G . Characterization of Sepik and Entebbe bat viruses closely related to yellow fever virus. Am J Trop Med Hyg. 2006; 75(6):1165-70. View

Felsenstein J . An alternating least squares approach to inferring phylogenies from pairwise distances. Syst Biol. 1997; 46(1):101-11. DOI: 10.1093/sysbio/46.1.101. View

Grard G, Moureau G, Charrel R, Lemasson J, Gonzalez J, Gallian P . Genetic characterization of tick-borne flaviviruses: new insights into evolution, pathogenetic determinants and taxonomy. Virology. 2006; 361(1):80-92. DOI: 10.1016/j.virol.2006.09.015. View

Cook S, Bennett S, Holmes E, de Chesse R, Moureau G, de Lamballerie X . Isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from Puerto Rico. J Gen Virol. 2006; 87(Pt 4):735-748. DOI: 10.1099/vir.0.81475-0. View

10.

Ramos H, Ribeiro H . Research on the mosquitoes of Angola (Diptera: culicidae) IX Genera Toxorhynchites Theo., 1901, Malaya Leicester, 1908, Ficalbia Theo, 1903, Coquillettidia Dyar, 1905, Mansonia Blanchard, 1901, Uranotaenia L. Arribálzaga, 1891, Aedeomyia Theo.,.... An Inst Hig Med Trop (Lisb). 1975; 3(1-4):111-41. View

11.

Stollar V, Thomas V . An agent in the Aedes aegypti cell line (Peleg) which causes fusion of Aedes albopictus cells. Virology. 1975; 64(2):367-77. DOI: 10.1016/0042-6822(75)90113-0. View

12.

Cook S, Holmes E . A multigene analysis of the phylogenetic relationships among the flaviviruses (Family: Flaviviridae) and the evolution of vector transmission. Arch Virol. 2005; 151(2):309-25. DOI: 10.1007/s00705-005-0626-6. View

13.

Medeiros D, Nunes M, Vasconcelos P, Chang G, Kuno G . Complete genome characterization of Rocio virus (Flavivirus: Flaviviridae), a Brazilian flavivirus isolated from a fatal case of encephalitis during an epidemic in Sao Paulo state. J Gen Virol. 2007; 88(Pt 8):2237-2246. DOI: 10.1099/vir.0.82883-0. View

14.

Nogueira R, Schatzmayr H, de Filippis A, Dos Santos F, da Cunha R, Coelho J . Dengue virus type 3, Brazil, 2002. Emerg Infect Dis. 2005; 11(9):1376-81. PMC: 3310608. DOI: 10.3201/eid1109.041043. View

15.

Chambers T, Hahn C, Galler R, Rice C . Flavivirus genome organization, expression, and replication. Annu Rev Microbiol. 1990; 44:649-88. DOI: 10.1146/annurev.mi.44.100190.003245. View

16.

Mackenzie J, Gubler D, Petersen L . Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat Med. 2004; 10(12 Suppl):S98-109. DOI: 10.1038/nm1144. View

17.

Hoshino K, Isawa H, Tsuda Y, Yano K, Sasaki T, Yuda M . Genetic characterization of a new insect flavivirus isolated from Culex pipiens mosquito in Japan. Virology. 2006; 359(2):405-14. DOI: 10.1016/j.virol.2006.09.039. View

18.

Diniz J, Nunes M, Travassos da Rosa A, Cruz A, De Souza W, Medeiros D . Characterization of two new rhabdoviruses isolated from midges (Culicoides SPP) in the Brazilian Amazon: proposed members of a new genus, Bracorhabdovirus. Arch Virol. 2006; 151(12):2519-27. DOI: 10.1007/s00705-006-0812-1. View

19.

Gaunt M, Sall A, de Lamballerie X, Falconar A, Dzhivanian T, Gould E . Phylogenetic relationships of flaviviruses correlate with their epidemiology, disease association and biogeography. J Gen Virol. 2001; 82(Pt 8):1867-1876. DOI: 10.1099/0022-1317-82-8-1867. View

20.

Shapiro A, Green T, Rao S, White S, Carner G, Mertens P . Morphological and molecular characterization of a Cypovirus (Reoviridae) from the mosquito Uranotaenia sapphirina (Diptera: Culicidae). J Virol. 2005; 79(15):9430-8. PMC: 1181557. DOI: 10.1128/JVI.79.15.9430-9438.2005. View