Amelioration of Nitrobenzene-induced Nephrotoxicity by the Ethanol Extract of the Herb Euphorbia Hirta

Overview

Journal Pharmacognosy Res

Date 2011 Oct 25

PMID 22022170

Citations 2

Authors

Subramanian Suganya

Dominic Sophia

Chinthamony Arul Raj

Muthaiyan Ahalliya Rathi

Lakshmanan Thirumoorthi

Periyasamy Meenakshi

Dugganaboyana Guru Kumar

Velliyur Kanniyapan Gopalakrishnan

Affiliations

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Abstract

Background: Euphorbia hirta (L.) (Euphorbiaceae) is a very popular herb amongst practitioners of traditional medicine and used in the treatment of female disorders, respiratory ailments, tumors, jaundice, digestive problems, wounds, etc. We aimed to evaluate the protective effect of E. hirta against nitrobenzene-induced nephrotoxicity in albino rats.

Materials And Methods: The nephroprotective activity of the ethanol extract of E. hirta (400 mg/kg body weight) was studied in nitrobenzene-induced albino rats (1000 mg/kg body weight). The activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and the levels of reduced glutathione (GSH), total thiols and vitamin C in the kidney tissues were determined. Histopathologic investigation was performed in the kidney tissue samples.

Results: Nitrobenzene administration significantly (P < 0.01) enhanced the lipid peroxidation and significantly (P < 0.05) depleted the levels of SOD, CAT, GPx, GST, GSH, total thiols and vitamin C. Treatment with the ethanol extract of E. hirta significantly normalized the antioxidant levels. The nephroprotective activity was also supported by histopathologic studies of kidney tissue.

Conclusion: The results indicate that the ethanol extract of E. hirta ameliorates renal dysfunction and could be used as an effective protector against nitrobenzene-induced nephrotoxicity, primarily through its antioxidant capacity.

Citing Articles

Minerals salt composition and secondary metabolites of Euphorbia hirta Linn., an antihyperglycemic plant.

Yvette Fofie N, Sanogo R, Coulibaly K, Kone-Bamba D Pharmacognosy Res. 2015; 7(1):7-13.

PMID: 25598628 PMC: 4285652. DOI: 10.4103/0974-8490.147131.

Systemic beneficial effects of Euphorbia besides its immunosuppressive effects.

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PMID: 22996085 DOI: 10.1007/s10787-012-0151-7.

References

Joy J, Nair C . Amelioration of cisplatin induced nephrotoxicity in Swiss albino mice by Rubia cordifolia extract. J Cancer Res Ther. 2008; 4(3):111-5. DOI: 10.4103/0973-1482.43139. View

El-Beshbishy H . Hepatoprotective effect of green tea (Camellia sinensis) extract against tamoxifen-induced liver injury in rats. J Biochem Mol Biol. 2005; 38(5):563-70. DOI: 10.5483/bmbrep.2005.38.5.563. View

Shanmugam K, Ramakrishna C, Mallikarjuna K, Reddy K . Protective effect of ginger against alcohol-induced renal damage and antioxidant enzymes in male albino rats. Indian J Exp Biol. 2010; 48(2):143-9. View

Wojcikowski K, Stevenson L, Leach D, Wohlmuth H, Gobe G . Antioxidant capacity of 55 medicinal herbs traditionally used to treat the urinary system: a comparison using a sequential three-solvent extraction process. J Altern Complement Med. 2007; 13(1):103-9. DOI: 10.1089/acm.2006.6122. View

Mansour H, Hafez H, Fahmy N . Silymarin modulates Cisplatin-induced oxidative stress and hepatotoxicity in rats. J Biochem Mol Biol. 2006; 39(6):656-61. DOI: 10.5483/bmbrep.2006.39.6.656. View

Omaye S, Turnbull J, SAUBERLICH H . Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods Enzymol. 1979; 62:3-11. DOI: 10.1016/0076-6879(79)62181-x. View

Perez Gutierrez R, Navarro Y . Antioxidant and hepatoprotective effects of the methanol extract of the leaves of Satureja macrostema. Pharmacogn Mag. 2010; 6(22):125-31. PMC: 2900060. DOI: 10.4103/0973-1296.62901. View

Sedlak J, Lindsay R . Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968; 25(1):192-205. DOI: 10.1016/0003-2697(68)90092-4. View

Cattley R, Everitt J, GROSS E, Moss O, Hamm Jr T, Popp J . Carcinogenicity and toxicity of inhaled nitrobenzene in B6C3F1 mice and F344 and CD rats. Fundam Appl Toxicol. 1994; 22(3):328-40. DOI: 10.1006/faat.1994.1039. View

10.

Habig W, Pabst M, Jakoby W . Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22):7130-9. View

11.

Jagetia G, Rajanikant G, Rao S, Shrinath Baliga M . Alteration in the glutathione, glutathione peroxidase, superoxide dismutase and lipid peroxidation by ascorbic acid in the skin of mice exposed to fractionated gamma radiation. Clin Chim Acta. 2003; 332(1-2):111-21. DOI: 10.1016/s0009-8981(03)00132-3. View

12.

Moron M, Depierre J, Mannervik B . Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta. 1979; 582(1):67-78. DOI: 10.1016/0304-4165(79)90289-7. View

13.

Hogberg J, Larson R, Kristoferson A, Orrenius S . NADPH-dependent reductase solubilized from microsomes by peroxidation and its activity. Biochem Biophys Res Commun. 1974; 56(3):836-42. DOI: 10.1016/0006-291x(74)90681-0. View

14.

Hsu C, Stedeford T, Okochi-Takada E, Ushijima T, Noguchi H, Muro-Cacho C . Framework analysis for the carcinogenic mode of action of nitrobenzene. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev. 2007; 25(2):155-84. DOI: 10.1080/10590500701399234. View

15.

Montilla P, Barcos M, Munoz M, Bujalance I, Munoz-Castaneda J, Tunez I . Red wine prevents brain oxidative stress and nephropathy in streptozotocin-induced diabetic rats. J Biochem Mol Biol. 2005; 38(5):539-44. DOI: 10.5483/bmbrep.2005.38.5.539. View

16.

Kumar S, Malhotra R, Kumar D . Euphorbia hirta: Its chemistry, traditional and medicinal uses, and pharmacological activities. Pharmacogn Rev. 2012; 4(7):58-61. PMC: 3249903. DOI: 10.4103/0973-7847.65327. View

17.

Ragheb A, Attia A, Eldin W, Elbarbry F, Gazarin S, Shoker A . The protective effect of thymoquinone, an anti-oxidant and anti-inflammatory agent, against renal injury: a review. Saudi J Kidney Dis Transpl. 2009; 20(5):741-52. View

18.

Misra H, Fridovich I . The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247(10):3170-5. View

19.

Sinha M, Manna P, Sil P . Amelioration of galactosamine-induced nephrotoxicity by a protein isolated from the leaves of the herb, Cajanus indicus L. BMC Complement Altern Med. 2007; 7:11. PMC: 1868763. DOI: 10.1186/1472-6882-7-11. View

20.

Ghosh A, Sil P . Anti-oxidative effect of a protein from Cajanus indicus L against acetaminophen-induced hepato-nephro toxicity. J Biochem Mol Biol. 2007; 40(6):1039-49. DOI: 10.5483/bmbrep.2007.40.6.1039. View