Characterization of Tritrichomonas Foetus Antigens by Use of Monoclonal Antibodies

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1990 Sep 1

PMID 2201645

Citations 16

Authors

J L Hodgson

D W Jones

P R Widders

L B Corbeil

Affiliations

Soon will be listed here.

Abstract

The specificity for and function of monoclonal antibodies against Tritrichomonas foetus were characterized. Four monoclonal antibodies generated by immunization of mice with live T. foetus were selected on the basis of enzyme-linked immunosorbent assay reactions. The approximate molecular masses of the predominant proteins were determined by Western blotting (immunoblotting). Monoclonal antibody TF3.8 recognized a predominant band at approximately 155 kilodaltons, whereas TF3.2 reacted with several bands. Monoclonal antibodies TF1.17 and TF1.15 recognized broad bands between 45 and 75 kilodaltons. The first two antibodies (TF3.8 and TF3.2) did not react with the surface of T. foetus, as determined by live-cell immunofluorescence, agglutination, and immobilization, whereas two other monoclonal antibodies (TF1.17 and TF1.15) did react with surface epitopes, as determined by these criteria. The latter two monoclonal antibodies also mediated complement-dependent killing of T. foetus and prevented of adherence of organisms to bovine vaginal epithelial cells. One antibody, TF1.15, also killed in the absence of complement. Since these functions are in vitro correlates of protection, the antigens recognized by these monoclonal antibodies may induce protective immunity.

Citing Articles

Systematic Review of Vaccine Strategies Against Tritrichomonas foetus Infection in Cattle: Insights, Challenges, and Prospects.

Santos J, Boe-Hansen G, Siddle H, Nguyen L, Raza A, McGowan M Parasite Immunol. 2025; 47(1):e70003.

PMID: 39838701 PMC: 11751591. DOI: 10.1111/pim.70003.

Comparative membrane proteomic analysis of Tritrichomonas foetus isolates.

Rivero M, Alonso A, Abdala M, Luque M, Carranza P, Coceres V Sci Rep. 2024; 14(1):17033.

PMID: 39043862 PMC: 11266394. DOI: 10.1038/s41598-024-67827-8.

Comparative aspects of immunity and vaccination in human and bovine trichomoniasis: a review.

Chapwanya A, Usman A, Irons P Trop Anim Health Prod. 2015; 48(1):1-7.

PMID: 26424377 DOI: 10.1007/s11250-015-0909-1.

Antibodies to Trichomonas vaginalis surface glycolipid.

Bastida-Corcuera F, Singh B, Gray G, Stamper P, Davuluri M, Schlangen K Sex Transm Infect. 2013; 89(6):467-72.

PMID: 23785040 PMC: 4685936. DOI: 10.1136/sextrans-2012-051013.

Murine models of vaginal trichomonad infections.

Cobo E, Eckmann L, Corbeil L Am J Trop Med Hyg. 2011; 85(4):667-73.

PMID: 21976570 PMC: 3183775. DOI: 10.4269/ajtmh.2011.11-0123.

References

Burgess D . Tritrichomonas foetus: preparation of monoclonal antibodies with effector function. Exp Parasitol. 1986; 62(2):266-74. DOI: 10.1016/0014-4894(86)90031-7. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Hall M, Huang J, Ota R, Redelman D, Hanks D, Taylor R . Characterization of Tritrichomonas foetus antigens, using bovine antiserum. Am J Vet Res. 1986; 47(12):2549-53. View

Kearney J, Radbruch A, Liesegang B, Rajewsky K . A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979; 123(4):1548-50. View

Parsonson I, Clark B, Dufty J . Early pathogenesis and pathology of Tritrichomonas foetus infection in virgin heifers. J Comp Pathol. 1976; 86(1):59-66. DOI: 10.1016/0021-9975(76)90028-1. View

Holbrook T, Boackle R, Vesely J, Parker B . Trichomonas vaginalis: alternative pathway activation of complement. Trans R Soc Trop Med Hyg. 1982; 76(4):473-5. DOI: 10.1016/0035-9203(82)90140-7. View

Corbeil L, Hodgson J, Jones D, Corbeil R, Widders P, Stephens L . Adherence of Tritrichomonas foetus to bovine vaginal epithelial cells. Infect Immun. 1989; 57(7):2158-65. PMC: 313855. DOI: 10.1128/iai.57.7.2158-2165.1989. View

Gillin F, Sher A . Activation of the alternative complement pathway by Trichomonas vaginalis. Infect Immun. 1981; 34(1):268-73. PMC: 350852. DOI: 10.1128/iai.34.1.268-273.1981. View

Kvasnicka W, Taylor R, Huang J, Hanks D, Tronstad R, Bosomworth A . Investigations of the incidence of bovine trichomoniasis in nevada and of the efficacy of immunizing cattle with vaccines containing Tritrichomonas foetus. Theriogenology. 1989; 31(5):963-71. DOI: 10.1016/0093-691x(89)90479-2. View

10.

Rae D . Impact of trichomoniasis on the cow-calf producer's profitability. J Am Vet Med Assoc. 1989; 194(6):771-5. View

11.

Woodward M, Young Jr W, Bloodgood R . Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. J Immunol Methods. 1985; 78(1):143-53. DOI: 10.1016/0022-1759(85)90337-0. View

12.

Huang J, Hanks D, Kvasnicka W, Hanks M, Hall M . Antigenic relationship among field isolates of Tritrichomonas foetus from cattle. Am J Vet Res. 1989; 50(7):1064-8. View

13.

LITTLEFIELD J . SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964; 145(3633):709-10. DOI: 10.1126/science.145.3633.709. View

14.

Gander J . Gel protein stains: glycoproteins. Methods Enzymol. 1984; 104:447-51. DOI: 10.1016/s0076-6879(84)04112-4. View

15.

Alderete J, Garza G . Identification and properties of Trichomonas vaginalis proteins involved in cytadherence. Infect Immun. 1988; 56(1):28-33. PMC: 259228. DOI: 10.1128/iai.56.1.28-33.1988. View

16.

Aydintug M, Leid R, Widders P . Antibody enhances killing of Tritrichomonas foetus by the alternative bovine complement pathway. Infect Immun. 1990; 58(4):944-8. PMC: 258565. DOI: 10.1128/iai.58.4.944-948.1990. View

17.

Skirrow S, BonDurant R . Induced Tritrichomonas foetus infection in beef heifers. J Am Vet Med Assoc. 1990; 196(6):885-9. View

18.

Goodger W, Skirrow S . Epidemiologic and economic analyses of an unusually long epizootic of trichomoniasis in a large California dairy herd. J Am Vet Med Assoc. 1986; 189(7):772-6. View

19.

Clark B, Dufty J, Parsonson I . Immunisation of bulls against trichomoniasis. Aust Vet J. 1983; 60(6):178-9. DOI: 10.1111/j.1751-0813.1983.tb05957.x. View

20.

Nedergaard J, Cannon B . Sodium-induced calcium release from mitochondria in brown adipose tissue. Proc Natl Acad Sci U S A. 1979; 76(5):2350-3. PMC: 383598. DOI: 10.1073/pnas.76.5.2350. View