Comparison of Potentials of Stem Cells Isolated from Tendon and Bone Marrow for Musculoskeletal Tissue Engineering

Overview

Journal Tissue Eng Part A

Specialties Anatomy & Histology
Biomedical Engineering
Biotechnology

Date 2011 Oct 21

PMID 22011320

Citations 99

Authors

Qi Tan

Pauline Po Yee Lui

Yun Feng Rui

Yin Mei Wong

Affiliations

Soon will be listed here.

Abstract

The use of tendon-derived stem cells (TDSCs) as a cell source for musculoskeletal tissue engineering has not been compared with that of bone marrow stromal cells (BMSC). This study compared the mesenchymal stem cell (MSC) and embryonic stem cells (ESC) markers, clonogenicity, proliferative capacity, and multilineage differentiation potential of rat TDSC and BMSC in vitro. The MSC and ESC marker profiles of paired TDSC and BMSC were compared using flow cytometry and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. Their clonogenicity and proliferative capacity were compared using colony-forming and 5-bromo-2'-deoxyuridine assays, respectively. The expression of tenogenic, osteogenic, and chondrogenic markers at basal state were examined using qRT-PCR. Their osteogenic, chondrogenic, and adipogenic differentiation potentials were compared using standard assays. TDSC and BMSC showed similar expression of CD90 and CD73. TDSC expressed higher levels of Oct4 than BMSC. TDSC exhibited higher clonogenicity, proliferated faster, and expressed higher tenomodulin, scleraxis, collagen 1 α 1 (Col1A1), decorin, alkaline phosphatase, Col2A1, and biglycan messenger RNA levels than BMSC. There was higher calcium nodule formation and osteogenic marker expression in TDSC than BMSC upon osteogenic induction. More chondrocyte-like cells and higher glycosaminoglycan deposition and chondrogenic marker expression were observed in TDSC than BMSC upon chondrogenic induction. There were more oil droplets and expression of an adipogenic marker in TDSC than BMSC upon adipogenic induction. TDSC expressed higher Oct4 levels, which was reported to positively regulate mesendodermal lineage differentiation, showed higher clonogenicity and proliferative capacity, and had greater tenogenic, osteogenic, chondrogenic, and adipogenic markers and differentiation potential than BMSC. TDSC might be a better cell source than BMSC for musculoskeletal tissue regeneration.

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References

Ge Z, Goh J, Lee E . Selection of cell source for ligament tissue engineering. Cell Transplant. 2005; 14(8):573-83. DOI: 10.3727/000000005783982819. View

Ni M, Lui P, Rui Y, Lee Y, Lee Y, Tan Q . Tendon-derived stem cells (TDSCs) promote tendon repair in a rat patellar tendon window defect model. J Orthop Res. 2011; 30(4):613-9. DOI: 10.1002/jor.21559. View

Lin T, Chang H, Wang K, Kao A, Chang C, Wen C . Isolation and identification of mesenchymal stem cells from human lipoma tissue. Biochem Biophys Res Commun. 2007; 361(4):883-9. DOI: 10.1016/j.bbrc.2007.07.116. View

Singh S, Dhaliwal N, Crawford R, Xiao Y . Cellular senescence and longevity of osteophyte-derived mesenchymal stem cells compared to patient-matched bone marrow stromal cells. J Cell Biochem. 2009; 108(4):839-50. DOI: 10.1002/jcb.22312. View

Baksh D, Yao R, Tuan R . Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells. 2007; 25(6):1384-92. DOI: 10.1634/stemcells.2006-0709. View

Tuan R, Boland G, Tuli R . Adult mesenchymal stem cells and cell-based tissue engineering. Arthritis Res Ther. 2003; 5(1):32-45. PMC: 154434. DOI: 10.1186/ar614. View

Lui P, Chan L, Lee Y, Fu S, Chan K . Sustained expression of proteoglycans and collagen type III/type I ratio in a calcified tendinopathy model. Rheumatology (Oxford). 2009; 49(2):231-9. DOI: 10.1093/rheumatology/kep384. View

Sakaguchi Y, Sekiya I, Yagishita K, Muneta T . Comparison of human stem cells derived from various mesenchymal tissues: superiority of synovium as a cell source. Arthritis Rheum. 2005; 52(8):2521-9. DOI: 10.1002/art.21212. View

Pittenger M, Mackay A, Beck S, Jaiswal R, Douglas R, Mosca J . Multilineage potential of adult human mesenchymal stem cells. Science. 1999; 284(5411):143-7. DOI: 10.1126/science.284.5411.143. View

10.

Lin L, Shen Q, Wei X, Hou Y, Xue T, Fu X . Comparison of osteogenic potentials of BMP4 transduced stem cells from autologous bone marrow and fat tissue in a rabbit model of calvarial defects. Calcif Tissue Int. 2009; 85(1):55-65. DOI: 10.1007/s00223-009-9250-x. View

11.

Deans T, Elisseeff J . Stem cells in musculoskeletal engineered tissue. Curr Opin Biotechnol. 2009; 20(5):537-44. DOI: 10.1016/j.copbio.2009.10.005. View

12.

Thomson M, Liu S, Zou L, Smith Z, Meissner A, Ramanathan S . Pluripotency factors in embryonic stem cells regulate differentiation into germ layers. Cell. 2011; 145(6):875-89. PMC: 5603300. DOI: 10.1016/j.cell.2011.05.017. View

13.

Kern S, Eichler H, Stoeve J, Kluter H, Bieback K . Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells. 2006; 24(5):1294-301. DOI: 10.1634/stemcells.2005-0342. View

14.

Yin Z, Chen X, Chen J, Shen W, Hieu Nguyen T, Gao L . The regulation of tendon stem cell differentiation by the alignment of nanofibers. Biomaterials. 2009; 31(8):2163-75. DOI: 10.1016/j.biomaterials.2009.11.083. View

15.

van Eijk F, Saris D, Riesle J, Willems W, van Blitterswijk C, Verbout A . Tissue engineering of ligaments: a comparison of bone marrow stromal cells, anterior cruciate ligament, and skin fibroblasts as cell source. Tissue Eng. 2004; 10(5-6):893-903. DOI: 10.1089/1076327041348428. View

16.

Rui Y, Lui P, Li G, Fu S, Lee Y, Chan K . Isolation and characterization of multipotent rat tendon-derived stem cells. Tissue Eng Part A. 2009; 16(5):1549-58. DOI: 10.1089/ten.TEA.2009.0529. View

17.

Matsubara T, Tsutsumi S, Pan H, Hiraoka H, Oda R, Nishimura M . A new technique to expand human mesenchymal stem cells using basement membrane extracellular matrix. Biochem Biophys Res Commun. 2003; 313(3):503-8. DOI: 10.1016/j.bbrc.2003.11.143. View

18.

Seo B, Miura M, Gronthos S, Bartold P, Batouli S, Brahim J . Investigation of multipotent postnatal stem cells from human periodontal ligament. Lancet. 2004; 364(9429):149-55. DOI: 10.1016/S0140-6736(04)16627-0. View

19.

Leo A, Grande D . Mesenchymal stem cells in tissue engineering. Cells Tissues Organs. 2006; 183(3):112-22. DOI: 10.1159/000095985. View

20.

Kolambkar Y, Peister A, Soker S, Atala A, Guldberg R . Chondrogenic differentiation of amniotic fluid-derived stem cells. J Mol Histol. 2007; 38(5):405-13. DOI: 10.1007/s10735-007-9118-1. View