Tendon-Derived Mesenchymal Stem Cells (TDSCs) As an In Vitro Model for Virological Studies in Wild Birds

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2023 Jul 29

PMID 37515142

Authors

Jose Rivas

Axel Dubois

Aude Blanquer

Mazarine Gerardy

Ute Ziegler

Martin H Groschup

Luc Grobet

Mutien-Marie Garigliany

Affiliations

Soon will be listed here.

Abstract

The use of wild animals in research is complicated due to the capture and housing conditions, as well as to legal aspects, making it difficult to develop in vivo and in vitro models for the study of pathologies that affect these species. Here we validate an in vitro model of tendon-derived mesenchymal cells (TDSC) from Eurasian blackbird () cadaveric samples. Through the expression of surface markers and the ability to differentiate into multiple lineages, the nature of the cells was confirmed. We then evaluated Mesenchymal Stem Cells (MSCs) as an infection model for the Usutu Flavivirus. To this aim, blackbird TDSCs were compared to Vero E6 cells, commonly used in Flavivirus studies. Both cells showed permissiveness to USUV infection as confirmed by immunocytochemistry. Moreover, TDSCs exhibited replication kinetics similar to, although slightly lower than, Vero E6, confirming these cells as a pertinent study model for the study of the pathogenesis of USUV. In this work, we isolated and characterized tendon-derived mesenchymal stem cells, which represent an interesting and convenient in vitro model for the study of wildlife species in laboratories.

Citing Articles

Correction: Rivas et al. Tendon-Derived Mesenchymal Stem Cells (TDSCs) as an In Vitro Model for Virological Studies in Wild Birds. 2023, , 1455.

Rivas J, Dubois A, Blanquer A, Gerardy M, Ziegler U, Groschup M Viruses. 2023; 15(12).

PMID: 38140697 PMC: 10695263. DOI: 10.3390/v15122283.

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