Mice Chronically Infected with Chimeric HIV Resist Peripheral and Brain Superinfection: a Model of Protective Immunity to HIV

Overview

Journal J Neuroimmune Pharmacol

Specialties Allergy & Immunology
Neurology
Pharmacology

Date 2011 Oct 12

PMID 21987348

Citations 26

Authors

Jennifer L Kelschenbach

Manisha Saini

Eran Hadas

Chao-Jiang Gu

Wei Chao

Galina Bentsman

Jessie P Hong

Tomas Hanke

Leroy R Sharer

Mary Jane Potash

David J Volsky

Affiliations

Soon will be listed here.

Abstract

Infection by some viruses induces immunity to reinfection, providing a means to identify protective epitopes. To investigate resistance to reinfection in an animal model of HIV disease and its control, we employed infection of mice with chimeric HIV, EcoHIV. When immunocompetent mice were infected by intraperitoneal (IP) injection of EcoHIV, they resisted subsequent secondary infection by IP injection, consistent with a systemic antiviral immune response. To investigate the potential role of these responses in restricting neurotropic HIV infection, we established a protocol for efficient EcoHIV expression in the brain following intracranial (IC) inoculation of virus. When mice were inoculated by IP injection and secondarily by IC injection, they also controlled EcoHIV replication in the brain. To investigate their role in EcoHIV antiviral responses, CD8+ T lymphocytes were isolated from spleens of EcoHIV infected and uninfected mice and adoptively transferred to isogenic recipients. Recipients of EcoHIV primed CD8+ cells resisted subsequent EcoHIV infection compared to recipients of cells from uninfected donors. CD8+ spleen cells from EcoHIV-infected mice also mounted modest but significant interferon-γ responses to two HIV Gag peptide pools. These findings suggest EcoHIV-infected mice may serve as a useful system to investigate the induction of anti-HIV protective immunity for eventual translation to human beings.

Citing Articles

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Implication of the Autophagy-Related Protein Beclin1 in the Regulation of EcoHIV Replication and Inflammatory Responses.

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Combined intranasal and intramuscular parainfluenza 5-, simian adenovirus ChAdOx1- and poxvirus MVA-vectored vaccines induce synergistically HIV-1-specific T cells in the mucosa.

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CCL2 is required for initiation but not persistence of HIV infection mediated neurocognitive disease in mice.

Kim B, Hadas E, Kelschenbach J, Chao W, Gu C, Potash M Sci Rep. 2023; 13(1):6577.

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