BAP31 and BiP Are Essential for Dislocation of SV40 from the Endoplasmic Reticulum to the Cytosol

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2011 Sep 28

PMID 21947079

Citations 99

Authors

Roger Geiger

Daniel Andritschke

Sarah Friebe

Fabian Herzog

Stefania Luisoni

Thomas Heger

Ari Helenius

Affiliations

Soon will be listed here.

Abstract

How non-enveloped viruses overcome host cell membranes is poorly understood. Here, we show that after endocytosis and transport to the endoplasmic reticulum (ER), but before crossing the ER membrane to the cytosol, incoming simian virus 40 particles are structurally remodelled leading to exposure of the amino-terminal sequence of the minor viral protein VP2. These hydrophobic sequences anchor the virus to membranes. A negatively charged residue, Glu 17, in the α-helical, membrane-embedded peptide is essential for infection, most likely by introducing an 'irregularity' recognized by the ER-associated degradation (ERAD) system for membrane proteins. Using a siRNA-mediated screen, the lumenal chaperone BiP and the ER-membrane protein BAP31 (both involved in ERAD) were identified as being essential for infection. They co-localized with the virus in discrete foci and promoted its ER-to-cytosol dislocation. Virus-like particles devoid of VP2 failed to cross the membrane. The results demonstrated that ERAD-factors assist virus transport across the ER membrane.

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