Controlled Translocation of Individual DNA Molecules Through Protein Nanopores with Engineered Molecular Brakes

Overview

Journal Nano Lett

Publisher American Chemical Society

Specialty Biotechnology

Date 2011 Jan 13

PMID 21222450

Citations 49

Authors

Marcela Rincon-Restrepo

Ellina Mikhailova

Hagan Bayley

Giovanni Maglia

Affiliations

Soon will be listed here.

Abstract

Protein nanopores may provide a cheap and fast technology to sequence individual DNA molecules. However, the electrophoretic translocation of ssDNA molecules through protein nanopores has been too rapid for base identification. Here, we show that the translocation of DNA molecules through the α-hemolysin protein nanopore can be slowed controllably by introducing positive charges into the lumen of the pore by site directed mutagenesis. Although the residual ionic current during DNA translocation is insufficient for direct base identification, we propose that the engineered pores might be used to slow down DNA in hybrid systems, for example, in combination with solid-state nanopores.

Citing Articles

Controlling DNA Fragments Translocation across Nanopores with the Synergic Use of Site-Directed Mutagenesis, pH-Dependent Charge Tuning, and Electroosmotic Flow.

Mereuta L, Bhatti H, Asandei A, Cimpanu A, Ying Y, Long Y ACS Appl Mater Interfaces. 2024; 16(30):40100-40110.

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Polymer Translocation and Nanopore Sequencing: A Review of Advances and Challenges.

Singh S, Chauhan K, Bharadwaj A, Kishore V, Laux P, Luch A Int J Mol Sci. 2023; 24(7).

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Functionalised nanopores: chemical and biological modifications.

Cairns-Gibson D, Cockroft S Chem Sci. 2022; 13(7):1869-1882.

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Herding cats: Label-based approaches in protein translocation through nanopore sensors for single-molecule protein sequence analysis.

Motone K, Cardozo N, Nivala J iScience. 2021; 24(9):103032.

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Nanopore sensing: A physical-chemical approach.

Robertson J, Ghimire M, Reiner J Biochim Biophys Acta Biomembr. 2021; 1863(9):183644.

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