Serodiagnosis of Viral Hepatitis A by a Modified Competitive Binding Radioimmunoassay for Immunoglobulin M Anti-hepatitis A Virus

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1979 Jan 1

PMID 219016

Citations 14

Authors

D W Bradley

H A Fields

K A McCaustland

J E MAYNARD

R H DECKER

R Whittington

L R Overby

Affiliations

Soon will be listed here.

Abstract

A competitive binding radioimmunoassay (CBA) for antibody to hepatitis A virus (HAV) was evaluated and compared with a standard solid-phase radioimmunoassay for anti-HAV, CBA was found to be sensitive and specific for the detection of anti-HAV, as demonstrated by the 98% concordance of CBA and solid-phase radioimmunoassay test results. The standard CBA test was modified for the differential detection of acute (immunoglobulin M) and convalescent (immunoglobulin G) anti-HAV by incorporation of a step in which immunoglobulin G anti-HAV was preferentially absorbed with S. aureus cells (protein A). The modified CBA test was shown to be capable of differentiating between acute- and convalescent-phase sera. The modified CBAM test was able to detect immunoglobulin M anti-HAV up to approximately 4 weeks after the onset of illness.

Citing Articles

Hepatitis A: old and new.

Cuthbert J Clin Microbiol Rev. 2001; 14(1):38-58.

PMID: 11148002 PMC: 88961. DOI: 10.1128/CMR.14.1.38-58.2001.

Serodiagnosis of acute hepatitis B virus infection by a modified competitive binding radioimmunoassay.

Schable C, MAYNARD J J Clin Microbiol. 1982; 16(5):973-5.

PMID: 7153350 PMC: 272515. DOI: 10.1128/jcm.16.5.973-975.1982.

Encephalitis during the prodromal stage of acute hepatitis A.

Hammond G, MacDougall B, Plummer F, Sekla L Can Med Assoc J. 1982; 126(3):269-70.

PMID: 7059901 PMC: 1862821.

Diagnosis of viral respiratory infections in the 1980s.

Friedman H, Forrer C Clin Lab Med. 1982; 2(2):383-91.

PMID: 6749390 PMC: 7173050.

Determination of immunoglobulin M antibodies against hepatitis B core antigen and hepatitis A virus by reorienting sucrose gradient high-speed centrifugation for diagnosis of acute viral-hepatitis.

Swenson P, Escobar M, Galen E, Carithers R J Clin Microbiol. 1981; 14(5):544-9.

PMID: 6273454 PMC: 273985. DOI: 10.1128/jcm.14.5.544-549.1981.

References

SZMUNESS W, Dienstag J, Purcell R, Prince A, Stevens C, Levine R . Hepatitis type A and hemodialysis: a seroepidemiologic study in 15 U.S. centers. Ann Intern Med. 1977; 87(1):8-12. DOI: 10.7326/0003-4819-87-1-8. View

Bradley D, Hollinger F, Hornbeck C, MAYNARD J . Isolation and characterization of hepatitis A virus. Am J Clin Pathol. 1976; 65(5 Suppl):876-89. View

Bradley D, Gravelle C, Cook E, Fields R, MAYNARD J . Cyclic excretion of hepatitis A virus in experimentally infected chimpanzees: biophysical characterization of the associated HAV particles. J Med Virol. 1977; 1(2):133-8. DOI: 10.1002/jmv.1890010206. View

Bradley D, McCaustland K, Schreeder M, Cook E, Gravelle C, MAYNARD J . Multiple buoyant densities of hepatitis A virus in cesium chloride gradients. J Med Virol. 1977; 1(3):219-26. DOI: 10.1002/jmv.1890010309. View

Bradley D, Hornbeck C, Cook E, MAYNARD J . Purification of hepatitis A virus from chimpanzee stools. J Virol. 1977; 22(1):228-31. PMC: 515704. DOI: 10.1128/JVI.22.1.228-231.1977. View

Bradley D, MAYNARD J, Hindman S, Hornbeck C, Fields H, McCaustland K . Serodiagnosis of viral hepatitis A: detection of acute-phase immunoglobulin M anti-hepatitis A virus by radioimmunoassay. J Clin Microbiol. 1977; 5(5):521-30. PMC: 274644. DOI: 10.1128/jcm.5.5.521-530.1977. View

Hollinger F, Bradley D, Dreesman G, Melnick J . Detection of viral hepatitis type A. Am J Clin Pathol. 1976; 65(5 Suppl):854-65. View

Frosner G, Overby L, Flehmig B, GERTH H, Haas H, DECKER R . Seroepidemiological investigation of patients and family contacts in an epidemic of hepatitis A. J Med Virol. 1977; 1(3):163-73. DOI: 10.1002/jmv.1890010303. View

Locarnini S, FERRIS A, LEHMANN N, Gust I . The antibody response following hepatitis A infection. Intervirology. 1977; 8(5):309-18. DOI: 10.1159/000148905. View

10.

Hall W, MUNDON F, Madden D, DeSouza D, Kane J . Distribution of antibody to hepatitis A antigen in a population of commerical plasma doners. J Clin Microbiol. 1977; 6(2):132-5. PMC: 274719. DOI: 10.1128/jcm.6.2.132-135.1977. View

11.

MAYNARD J, Bradley D, Hornbeck C, Fields R, DOTO I, Hollinger F . Preliminary serologic studies of antibody to hepatitis A virus in populations in the United States. J Infect Dis. 1976; 134(5):528-30. DOI: 10.1093/infdis/134.5.528. View

12.

SZMUNESS W, Purcell R, Dienstag J, Stevens C . Antibody to hepatitis A antigen in institutionalized mentally retarded patients. JAMA. 1977; 237(16):1702-5. View

13.

Dienstag J, Alling D, Purcell R . Quantitation of antibody to hepatitis A antigen by immune electron microscopy. Infect Immun. 1976; 13(4):1209-13. PMC: 420740. DOI: 10.1128/iai.13.4.1209-1213.1976. View

14.

Purcell R, Wong D, Moritsugu Y, Dienstag J, Routenberg J, BOGGS J . A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody. J Immunol. 1976; 116(2):349-56. View

15.

HILLEMAN M, Provost P, MILLER W, VILLAREJOS V, ITTENSOHN O, McALEER W . Immune adherence and complement-fixation tests for human hepatitis A. Diagnostic and epidemiologic investigations. Dev Biol Stand. 1975; 30:383-9. View

16.

Gravelle C, Hornbeck C, MAYNARD J, Schable C, Cook E, Bradley D . Hepatitis A: report of a common-source outbreak with recovery of a possible etiologic agent. II. Laboratory studies. J Infect Dis. 1975; 131(2):167-71. DOI: 10.1093/infdis/131.2.167. View

17.

MILLER W, Provost P, McALEER W, ITTENSOHN O, VILLAREJOS V, HILLEMAN M . Specific immune adherence assay for human hepatitis A antibody application to diagnostic and epidemiologic investigations. Proc Soc Exp Biol Med. 1975; 149(1):254-61. DOI: 10.3181/00379727-149-38783. View

18.

SZMUNESS W, Dienstag J, Purcell R, Harley E, Stevens C, Wong D . Distribution of antibody to hepatitis a antigen in urban adult populations. N Engl J Med. 1976; 295(14):755-9. DOI: 10.1056/NEJM197609302951404. View

19.

Provost P, ITTENSOHN O, VILLAREJOS V, HILLEMAN M . A specific complement-fixation test for human hepatitis a employing CR326 virus antigen. Diagnosis and epidemiology. Proc Soc Exp Biol Med. 1975; 148(4):962-9. DOI: 10.3181/00379727-148-38669. View