Two Separate Ni(2+) -sensitive Voltage-gated Ca(2+) Channels Modulate Transretinal Signalling in the Isolated Murine Retina

Overview

Journal Acta Ophthalmol

Specialty Ophthalmology

Date 2011 Sep 3

PMID 21883984

Citations 5

Authors

Maged Alnawaiseh

Walid Albanna

Chien-Chang Chen

Kevin P Campbell

Jurgen Hescheler

Matthias Luke

Toni Schneider

Affiliations

Soon will be listed here.

Abstract

Purpose: Light-evoked responses from vertebrate retinas were recorded as an electroretinogram (ERG). The b-wave is the most prominent component of the ERG, and in the bovine retina its NiCl(2) -sensitive component was attributed to reciprocal signalling by pharmacoresistant R-type voltage-gated Ca(2+) channels, which similar to other voltage-dependent Ca(2+) channels trigger and control neurotransmitter release. The murine retina has the great advantage that the effect of gene inactivation for Ni(2+) -sensitive Ca(2+) channels can be analysed to prove or disprove that any of these Ca(2+) channels is involved in retinal signalling.

Methods: Superfused retinas from different murine genotypes lacking either one or both highly Ni(2+) -sensitive voltage-gated Ca(2+) channels were used to record their ex vivo ERGs.

Results: The isolated retinas from mice lacking Ca(v)2.3 R-type or Ca(v)3.2 T-type or both voltage-gated Ca(2+) channels were superfused with a NiCl(2) (15 μm) containing nutrient solution. The change in the b-wave amplitude and implicit time, caused by NiCl(2), was calculated as a difference spectrum and compared to data from control animals. From the results, it can be deduced that Ca(v)2.3 contributes rather to a later component in the b-wave response, while in the absence of Ca(v)3.2 the gain of Ni(2+) -mediated increase in the b-wave amplitude is significantly increased, probably due to a loss of reciprocal inhibition to photoreceptors. Thus, each of the Ni(2+)-sensitive Ca(2+) channels contributes to specific features of the b-wave response.

Conclusion: Both high-affinity Ni(2+)-sensitive Ca(2+) channels contribute to transretinal signalling. Based on the results from the double knockout mice, additional targets for NiCl(2) must contribute to transretinal signalling, which will be most important for the structurally similar physiologically more important heavy metal cation Zn(2+).

Citing Articles

Retinal Vessel Responses to Flicker Stimulation Are Impaired in Ca 2.3-Deficient Mice-An Evaluation Using Retinal Vessel Analysis (RVA).

Neumaier F, Kotliar K, Haeren R, Temel Y, Luke J, Seyam O Front Neurol. 2021; 12:659890.

PMID: 33927686 PMC: 8076560. DOI: 10.3389/fneur.2021.659890.

Submicromolar copper (II) ions stimulate transretinal signaling in the isolated retina from wild type but not from Ca2.3-deficient mice.

Luke J, Neumaier F, Alpdogan S, Hescheler J, Schneider T, Albanna W BMC Ophthalmol. 2020; 20(1):182.

PMID: 32375703 PMC: 7201970. DOI: 10.1186/s12886-020-01451-8.

Unconjugated bilirubin modulates neuronal signaling only in wild-type mice, but not after ablation of the R-type/Ca 2.3 voltage-gated calcium channel.

Albanna W, Neumaier F, Luke J, Kotliar K, Conzen C, Lindauer U CNS Neurosci Ther. 2017; 24(3):222-230.

PMID: 29274300 PMC: 6489700. DOI: 10.1111/cns.12791.

Evaluation of micro Electroretinograms Recorded with Multiple Electrode Array to Assess Focal Retinal Function.

Fujii M, Sunagawa G, Kondo M, Takahashi M, Mandai M Sci Rep. 2016; 6:30719.

PMID: 27480484 PMC: 4969741. DOI: 10.1038/srep30719.

Low concentrations of ethanol but not of dimethyl sulfoxide (DMSO) impair reciprocal retinal signal transduction.

Siapich S, Akhtar I, Hescheler J, Schneider T, Luke M Graefes Arch Clin Exp Ophthalmol. 2015; 253(10):1713-9.

PMID: 26104874 DOI: 10.1007/s00417-015-3070-7.

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