Development of a Multiplex PCR Assay Targeting O-antigen Modification Genes for Molecular Serotyping of Shigella Flexneri

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2011 Sep 2

PMID 21880974

Citations 32

Authors

Qiangzheng Sun

Ruiting Lan

Yiting Wang

Ailan Zhao

Shaomin Zhang

Jianping Wang

Yan Wang

Shengli Xia

Dong Jin

Zhigang Cui

Hongqing Zhao

Zhenjun Li

Changyun Ye

Shuxia Zhang

Huaiqi Jing

Jianguo Xu

Affiliations

Soon will be listed here.

Abstract

Shigella flexneri is the major Shigella species that causes diarrheal disease in developing countries. It is further subdivided into 15 serotypes based on O-antigen structure. Serotyping of S. flexneri is important for epidemiological purposes. In this study, we developed a multiplex PCR assay targeting the O-antigen synthesis gene wzx and the O-antigen modification genes gtrI, gtrIC, gtrII, oac, gtrIV, gtrV, and gtrX for molecular serotyping of S. flexneri. The multiplex PCR assay contained eight sets of specific PCRs in a single tube and can identify 14 of the 15 serotypes (the exception being serotype Xv) of S. flexneri recognized thus far. A nearly perfect concordance (97.8%) between multiplex PCR assay and slide agglutination was observed when 358 S. flexneri strains of various serotypes were analyzed, except that 8 strains were carrying additional cryptic and/or defective serotype-specific genes. The multiplex PCR assay provides a rapid and specific method for the serotype identification of S. flexneri.

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