In Vivo Measurement of Glioma-induced Vascular Permeability

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2011 Aug 30

PMID 21874468

Citations 12

Authors

Jisook Lee

Andrew Baird

Brian P Eliceiri

Affiliations

Soon will be listed here.

Abstract

The normal blood-brain barrier (BBB) consists of tight interendothelial cell junctions and adjacent astrocyte end feet separated by a basal lamina surrounding the endothelium. The interactions between the different cell types of BBB are disrupted in distinct patterns in the microenvironment of glioma. Malignant gliomas infiltrate the surrounding normal brain parenchyma; a process associated with vascular permeability (VP) and breakdown of the BBB. Herein, we describe methods to quantitatively measure glioma-induced vascular permeability, utilizing an orthotopic xenograft model of glioma.

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References

Iadecola C . Neurovascular regulation in the normal brain and in Alzheimer's disease. Nat Rev Neurosci. 2004; 5(5):347-60. DOI: 10.1038/nrn1387. View

Lampson L, Lampson M, Dunne A . Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in.... Cancer Res. 1993; 53(1):176-82. View

Abbott N, Ronnback L, Hansson E . Astrocyte-endothelial interactions at the blood-brain barrier. Nat Rev Neurosci. 2005; 7(1):41-53. DOI: 10.1038/nrn1824. View

Zhang X, Li X, Wu J, Gao D, Liang J, Liu X . Experiment and observation on invasion of brain glioma in vivo. J Clin Neurosci. 2003; 9(6):668-71. DOI: 10.1054/jocn.2002.1140. View

Neuwelt E, Rapoport S . Modification of the blood-brain barrier in the chemotherapy of malignant brain tumors. Fed Proc. 1984; 43(2):214-9. View