Aberrant Antigenic Expression in Extranodal NK/T-cell Lymphoma: a Multi-parameter Study from Thailand

Overview

Journal Diagn Pathol

Publisher Biomed Central

Specialty Pathology

Date 2011 Aug 27

PMID 21867533

Citations 22

Authors

Tawatchai Pongpruttipan

Tanawan Kummalue

Anan Bedavanija

Archrob Khuhapinant

Koichi Ohshima

Fumiko Arakawa

Daisuke Niino

Sanya Sukpanichnant

Affiliations

Soon will be listed here.

Abstract

Background: Extranodal NK/T-cell lymphoma, nasal type (ENKTL) is not common worldwide, but it is the most common T- and NK-cell lymphomas in many Asian countries. Immunophenotypic profiles were studied based on limited series. The authors, therefore, studied on ENKTL according to characterize immunophenotypic profiles as well as the distribution of EBV subtype and LMP-1 gene deletion.

Methods: By using tissue microarray (TMA), immunohistochemical study and EBV encoded RNA (EBER) in situ hybridization were performed. T-cell receptor (TCR) gene rearrangement, EBV subtyping, and LMP-1 gene deletion were studied on the available cases.

Results: There were 22 cases eligible for TMA. ENKTL were positive for CD3 (91%), CD5 (9%), CD7 (32%), CD4 (14%), CD56 (82%), TIA-1 (100%), granzyme B (95%), perforin (86%), CD45 (83%), CD30 (75%), Oct2 (25%), and IRF4/MUM1 (33%). None of them was positive for βF1, CD8, or CD57. TCR gene rearrangement was negative in all 18 tested cases. EBV was subtype A in all 15 tested cases, with 87% deleted LMP-1 gene. Cases lacking perforin expression demonstrated a significantly poorer survival outcome (p = 0.008).

Conclusions: The present study demonstrated TIA-1 and EBER as the two most sensitive markers. There were a few CD3 and/or CD56 negative cases noted. Interestingly, losses of CD45 and/or CD7 were not uncommon while Oct2 and IRF4/MUM1 could be positive in a subset of cases. Based on the present study in conjunction with the literature review, determination of PCR-based TCR gene rearrangement analysis might not be a useful technique for making diagnosis of ENKTL.

Citing Articles

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The Pathologic and Genetic Characteristics of Extranodal NK/T-Cell Lymphoma.

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Extranodal NK/T cell lymphoma, nasal type: a rare diagnosis with common nasal presentation.

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Primary pulmonary extranodal natural killer/T-cell lymphoma (ENKTL), nasal type: Two case reports and literature review.

Hu Q, Xu L, Zhang X, Wang J, Zhou Z Medicine (Baltimore). 2020; 99(26):e20822.

PMID: 32590768 PMC: 7328944. DOI: 10.1097/MD.0000000000020822.

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