Triacylglycerol Profiling of Marine Microalgae by Mass Spectrometry

Overview

Journal J Lipid Res

Publisher Elsevier

Specialty Biochemistry

Date 2011 Aug 16

PMID 21840867

Citations 17

Authors

Megan A Danielewicz

Lisa A Anderson

Annaliese K Franz

Affiliations

Soon will be listed here.

Abstract

We present a method for the determination of triacylglycerol (TAG) profiles of oleaginous saltwater microalgae relevant for the production of biofuels, bioactive lipids, and high-value lipid-based chemical precursors. We describe a technique to remove chlorophyll using quick, simple solid phase extraction (SPE) and directly compare the intact TAG composition of four microalgae species (Phaeodactylum tricornutum, Nannochloropsis salina, Nannochloropsis oculata, and Tetraselmis suecica) using MALDI time-of-flight (TOF) mass spectrometry (MS), ESI linear ion trap-orbitrap (LTQ Orbitrap) MS, and ¹H NMR spectroscopy. Direct MS analysis is particularly effective to compare the polyunsaturated fatty acid (PUFA) composition for triacylglycerols because oxidation can often degrade samples upon derivatization. Using these methods, we observed that T. suecica contains significant PUFA levels with respect to other microalgae. This method is applicable for high-throughput MS screening of microalgae TAG profiles and may aid in the commercial development of biofuels.

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References

Lepage G, Roy C . Direct transesterification of all classes of lipids in a one-step reaction. J Lipid Res. 1986; 27(1):114-20. View

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

Fuchs B, Suss R, Schiller J . An update of MALDI-TOF mass spectrometry in lipid research. Prog Lipid Res. 2010; 49(4):450-75. DOI: 10.1016/j.plipres.2010.07.001. View

Emerson B, Gidden J, Lay Jr J, Durham B . A rapid separation technique for overcoming suppression of triacylglycerols by phosphatidylcholine using MALDI-TOF MS. J Lipid Res. 2010; 51(8):2428-34. PMC: 2903816. DOI: 10.1194/jlr.D003798. View

Vieler A, Wilhelm C, Goss R, Suss R, Schiller J . The lipid composition of the unicellular green alga Chlamydomonas reinhardtii and the diatom Cyclotella meneghiniana investigated by MALDI-TOF MS and TLC. Chem Phys Lipids. 2007; 150(2):143-55. DOI: 10.1016/j.chemphyslip.2007.06.224. View

Suzuki T, Midonoya H, Shioi Y . Analysis of chlorophylls and their derivatives by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. Anal Biochem. 2009; 390(1):57-62. DOI: 10.1016/j.ab.2009.04.005. View

Miao X, Wu Q . High yield bio-oil production from fast pyrolysis by metabolic controlling of Chlorella protothecoides. J Biotechnol. 2004; 110(1):85-93. DOI: 10.1016/j.jbiotec.2004.01.013. View

PIE A, Giner A . Solvents for thin layer chromatography of blood serum lipids. Nature. 1966; 212(5060):402-3. DOI: 10.1038/212402a0. View

Hsu F, Turk J . Electrospray ionization multiple-stage linear ion-trap mass spectrometry for structural elucidation of triacylglycerols: assignment of fatty acyl groups on the glycerol backbone and location of double bonds. J Am Soc Mass Spectrom. 2010; 21(4):657-69. PMC: 2847672. DOI: 10.1016/j.jasms.2010.01.007. View

10.

Nicola A, Gusev A, Proctor A, Jackson E, Hercules D . Application of the fast-evaporation sample preparation method for improving quantification of angiotensin II by matrix-assisted laser desorption/ionization. Rapid Commun Mass Spectrom. 1995; 9(12):1164-71. DOI: 10.1002/rcm.1290091216. View

11.

Lay Jr J, Liyanage R, Durham B, Brooks J . Rapid characterization of edible oils by direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using triacylglycerols. Rapid Commun Mass Spectrom. 2006; 20(6):952-8. DOI: 10.1002/rcm.2394. View

12.

Deluca S, Sarver E, Harrington P, Voorhees K . Direct analysis of bacterial fatty acids by Curie-point pyrolysis tandem mass spectrometry. Anal Chem. 1990; 62(14):1465-72. DOI: 10.1021/ac00213a022. View

13.

Guschina I, Harwood J . Lipids and lipid metabolism in eukaryotic algae. Prog Lipid Res. 2006; 45(2):160-86. DOI: 10.1016/j.plipres.2006.01.001. View

14.

Chen W, Zhang C, Song L, Sommerfeld M, Hu Q . A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J Microbiol Methods. 2009; 77(1):41-7. DOI: 10.1016/j.mimet.2009.01.001. View

15.

Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M . Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J. 2008; 54(4):621-39. DOI: 10.1111/j.1365-313X.2008.03492.x. View

16.

Murphy R, Fiedler J, Hevko J . Analysis of nonvolatile lipids by mass spectrometry. Chem Rev. 2001; 101(2):479-526. DOI: 10.1021/cr9900883. View

17.

Han X, Gross R . Quantitative analysis and molecular species fingerprinting of triacylglyceride molecular species directly from lipid extracts of biological samples by electrospray ionization tandem mass spectrometry. Anal Biochem. 2001; 295(1):88-100. DOI: 10.1006/abio.2001.5178. View

18.

Robles Medina A, Grima E, Gimenez Gimenez A, Ibanez Gonzalez M . Downstream processing of algal polyunsaturated fatty acids. Biotechnol Adv. 2003; 16(3):517-80. DOI: 10.1016/s0734-9750(97)00083-9. View

19.

Annarao S, Sidhu O, Roy R, Tuli R, Khetrapal C . Lipid profiling of developing Jatropha curcas L. seeds using (1)H NMR spectroscopy. Bioresour Technol. 2008; 99(18):9032-5. DOI: 10.1016/j.biortech.2008.04.023. View

20.

Hara A, RADIN N . Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem. 1978; 90(1):420-6. DOI: 10.1016/0003-2697(78)90046-5. View