Proteolipidic Composition of Exosomes Changes During Reticulocyte Maturation

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2011 Aug 11

PMID 21828046

Citations 87

Authors

Kevin Carayon

Karima Chaoui

Elsa Ronzier

Ikrame Lazar

Justine Bertrand-Michel

Veronique Roques

Stephanie Balor

Francois Terce

Andre Lopez

Laurence Salome

Etienne Joly

Affiliations

Soon will be listed here.

Abstract

During the orchestrated process leading to mature erythrocytes, reticulocytes must synthesize large amounts of hemoglobin, while eliminating numerous cellular components. Exosomes are small secreted vesicles that play an important role in this process of specific elimination. To understand the mechanisms of proteolipidic sorting leading to their biogenesis, we have explored changes in the composition of exosomes released by reticulocytes during their differentiation, in parallel to their physical properties. By combining proteomic and lipidomic approaches, we found dramatic alterations in the composition of the exosomes retrieved over the course of a 7-day in vitro differentiation protocol. Our data support a previously proposed model, whereby in reticulocytes the biogenesis of exosomes involves several distinct mechanisms for the preferential recruitment of particular proteins and lipids and suggest that the respective prominence of those pathways changes over the course of the differentiation process.

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