Cyanidin-3-rutinoside Alleviates Postprandial Hyperglycemia and Its Synergism with Acarbose by Inhibition of Intestinal α-glucosidase

Overview

Journal J Clin Biochem Nutr

Specialty Biochemistry

Date 2011 Jul 19

PMID 21765605

Citations 33

Authors

Sirichai Adisakwattana

Sirintorn Yibchok-anun

Piyawan Charoenlertkul

Natthakarn Wongsasiripat

Affiliations

Soon will be listed here.

Abstract

The inhibitory activity on intestinal α-glucosidase by cyanidin-3-rutinoside was examined in vitro and in vivo. The IC(50) values of cyanidin-3-rutinoside against intestinal maltase, and sucrase were 2,323 ± 14.8 and 250.2 ± 8.1 µM, respectively. The kinetic analysis revealed that intestinal sucrase was inhibited by cyanidin-3-rutinoside in a mixed-type manner. The synergistic inhibition also found in combination of cyanidin-3-rutinoside with acarbose against intestinal maltase and sucrase. The oral administration of cyanidin-3-rutinoside (100 and 300 mg/kg) plus maltose or sucrose to normal rats, postprandial plasma glucose was markedly suppressed at 30-90 min after loading. Furthermore, the normal rats treated with acarbose and cyanidin-3-rutinoside (30 mg/kg) showed greater reduction of postprandial plasma glucose than the group treated with acarbose alone. These results suggest that cyanidin-3-rutinoside retards absorption of carbohydrates by inhibition of α-glucosidase which may be useful as a potential inhibitor for prevention and treatment of diabetes mellitus.

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References

Hanefeld M, Fischer S, Schulze J, Spengler M, Wargenau M, Schollberg K . Therapeutic potentials of acarbose as first-line drug in NIDDM insufficiently treated with diet alone. Diabetes Care. 1991; 14(8):732-7. DOI: 10.2337/diacare.14.8.732. View

Raptis S, Dimitriadis G . Oral hypoglycemic agents: insulin secretagogues, alpha-glucosidase inhibitors and insulin sensitizers. Exp Clin Endocrinol Diabetes. 2001; 109 Suppl 2:S265-87. DOI: 10.1055/s-2001-18588. View

Galvano F, La Fauci L, Vitaglione P, Fogliano V, Vanella L, Felgines C . Bioavailability, antioxidant and biological properties of the natural free-radical scavengers cyanidin and related glycosides. Ann Ist Super Sanita. 2008; 43(4):382-93. View

Aymoto Hassimotto N, Genovese M, Lajolo F . Absorption and metabolism of cyanidin-3-glucoside and cyanidin-3-rutinoside extracted from wild mulberry (Morus nigra L.) in rats. Nutr Res. 2008; 28(3):198-207. DOI: 10.1016/j.nutres.2007.12.012. View

Chiasson J . Acarbose for the prevention of diabetes, hypertension, and cardiovascular disease in subjects with impaired glucose tolerance: the Study to Prevent Non-Insulin-Dependent Diabetes Mellitus (STOP-NIDDM) Trial. Endocr Pract. 2006; 12 Suppl 1:25-30. DOI: 10.4158/EP.12.S1.25. View

Sasaki R, Nishimura N, Hoshino H, Isa Y, Kadowaki M, Ichi T . Cyanidin 3-glucoside ameliorates hyperglycemia and insulin sensitivity due to downregulation of retinol binding protein 4 expression in diabetic mice. Biochem Pharmacol. 2007; 74(11):1619-27. DOI: 10.1016/j.bcp.2007.08.008. View

Ceriello A . Postprandial hyperglycemia and diabetes complications: is it time to treat?. Diabetes. 2004; 54(1):1-7. DOI: 10.2337/diabetes.54.1.1. View

Cho S, Roman G, Yeboah F, Konishi Y . The road to advanced glycation end products: a mechanistic perspective. Curr Med Chem. 2007; 14(15):1653-71. DOI: 10.2174/092986707780830989. View

Matsui T, Ebuchi S, Kobayashi M, Fukui K, Sugita K, Terahara N . Anti-hyperglycemic effect of diacylated anthocyanin derived from Ipomoea batatas cultivar Ayamurasaki can be achieved through the alpha-glucosidase inhibitory action. J Agric Food Chem. 2002; 50(25):7244-8. DOI: 10.1021/jf025913m. View

10.

Misra A, Khurana L . Obesity and the metabolic syndrome in developing countries. J Clin Endocrinol Metab. 2008; 93(11 Suppl 1):S9-30. DOI: 10.1210/jc.2008-1595. View

11.

Adisakwattana S, Ngamrojanavanich N, Kalampakorn K, Tiravanit W, Roengsumran S, Yibchok-anun S . Inhibitory activity of cyanidin-3-rutinoside on alpha-glucosidase. J Enzyme Inhib Med Chem. 2004; 19(4):313-6. DOI: 10.1080/14756360409162443. View

12.

Chiasson J, Josse R, Gomis R, Hanefeld M, Karasik A, Laakso M . Acarbose for prevention of type 2 diabetes mellitus: the STOP-NIDDM randomised trial. Lancet. 2002; 359(9323):2072-7. DOI: 10.1016/S0140-6736(02)08905-5. View

13.

Lebovitz H . Postprandial hyperglycaemic state: importance and consequences. Diabetes Res Clin Pract. 1998; 40 Suppl:S27-8. View

14.

Ghosh D, Konishi T . Anthocyanins and anthocyanin-rich extracts: role in diabetes and eye function. Asia Pac J Clin Nutr. 2007; 16(2):200-8. View

15.

Mulabagal V, Lang G, DeWitt D, Dalavoy S, Nair M . Anthocyanin content, lipid peroxidation and cyclooxygenase enzyme inhibitory activities of sweet and sour cherries. J Agric Food Chem. 2009; 57(4):1239-46. DOI: 10.1021/jf8032039. View

16.

Tulio Jr A, Reese R, Wyzgoski F, Rinaldi P, Fu R, Scheerens J . Cyanidin 3-rutinoside and cyanidin 3-xylosylrutinoside as primary phenolic antioxidants in black raspberry. J Agric Food Chem. 2008; 56(6):1880-8. DOI: 10.1021/jf072313k. View

17.

Jayaprakasam B, Vareed S, Olson L, Nair M . Insulin secretion by bioactive anthocyanins and anthocyanidins present in fruits. J Agric Food Chem. 2005; 53(1):28-31. DOI: 10.1021/jf049018+. View

18.

Adisakwattana S, Jiphimai P, Prutanopajai P, Chanathong B, Sapwarobol S, Ariyapitipan T . Evaluation of alpha-glucosidase, alpha-amylase and protein glycation inhibitory activities of edible plants. Int J Food Sci Nutr. 2010; 61(3):295-305. DOI: 10.3109/09637480903455963. View

19.

Chen P, Chu S, Chiou H, Kuo W, Chiang C, Hsieh Y . Mulberry anthocyanins, cyanidin 3-rutinoside and cyanidin 3-glucoside, exhibited an inhibitory effect on the migration and invasion of a human lung cancer cell line. Cancer Lett. 2005; 235(2):248-59. DOI: 10.1016/j.canlet.2005.04.033. View

20.

Adisakwattana S, Chantarasinlapin P, Thammarat H, Yibchok-anun S . A series of cinnamic acid derivatives and their inhibitory activity on intestinal alpha-glucosidase. J Enzyme Inhib Med Chem. 2009; 24(5):1194-200. DOI: 10.1080/14756360902779326. View