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Strategy for Modular Tagged High-throughput Amplicon Sequencing

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 2011 Jul 19
PMID 21764953
Citations 15
Authors
Daniel Aguirre de Carcer
Stuart E Denman
Chris McSweeney
Mark Morrison
Affiliations
Soon will be listed here.
Abstract

The use and validation of a strategy that allows a universal set of bar-coded sequencing primers to be appended to an amplified PCR product is described. The strategy allows a modular approach, in that the same bar code can be used with two or more target-specific primer sets, even simultaneously.

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