Cloning and Characterization of a Drosophila Serotonin Receptor That Activates Adenylate Cyclase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Nov 1

PMID 2174167

Citations 62

Authors

P Witz

N Amlaiky

J L Plassat

L Maroteaux

E Borrelli

R Hen

Affiliations

Soon will be listed here.

Abstract

Using a strategy based on nucleotide sequence homology between genes encoding receptors that interact with guanine nucleotide-binding proteins, we have isolated Drosophila genomic and cDNA clones encoding a functional serotonin receptor (5HT-dro receptor). This protein is expressed predominantly in Drosophila heads and exhibits highest homology with the human 5HT1A receptor. The predicted structure of the 5HT-dro receptor reveals two unusual features: (i) eight putative transmembrane domains instead of the expected seven and (ii) a Gly-Ser repeat that is a potential glycosaminoglycan attachment site. When stably introduced into mouse NIH 3T3 cells, the 5HT-dro receptor activates adenylate cyclase in response to serotonin and is inhibited by serotonin receptor antagonists such as dihydroergocryptine. The 5HT-dro receptor or closely related receptors might be responsible for the serotonin-sensitive cyclase that has been suggested to play a role in learning and modulation of circadian rhythm in a number of invertebrate systems.

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