In Vivo MicroRNA-155 Expression Influences Antigen-specific T Cell-mediated Immune Responses Generated by DNA Vaccination

Overview

Journal Cell Biosci

Publisher Biomed Central

Specialty Biology

Date 2011 Jun 30

PMID 21711593

Citations 12

Authors

Chih-Ping Mao

Liangmei He

Ya-Chea Tsai

Shiwen Peng

Tae Heung Kang

Xiaowu Pang

Archana Monie

Chien-Fu Hung

T-C Wu

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNA (miRNA) molecules are potent mediators of post-transcriptional gene silencing that are emerging to be critical in the regulation of innate and adaptive immunity.

Results: Here we report that miR-155--an oncogenic miRNA with important function in the mammalian immune system--is induced in dendritic cells (DCs) upon maturation and potentially attenuates their ability to activate T cells. Biolistic epidermal transfection with DNA encoding miR-155 suppressed the induction of antigen-specific T cell-mediated immunity, whereas reduction of endogenous miR-155 by a partially complementary antisense sequence reversed this effect. Because DCs represent a significant component of epidermal tissue and are among the most potent of antigen-presenting cells, the inhibitory actions of miR-155 could be mediated through this subset of cells.

Conclusions: These results suggest that miR-155 may repress the expression of key molecules involved in lymph node migration, antigen presentation, or T cell activation in DCs, and thus forms part of a negative regulatory pathway that dampens the generation of T cell-mediated immune responses. Modulation of miR-155 expression in epidermis therefore represents a potentially promising form of gene therapy for the control of diseases ranging from autoimmunity to cancer and viral infection.

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