Levels of MRNA for a Putative Kainate Receptor Are Affected by Seizures

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Oct 1

PMID 2170985

Citations 6

Authors

C Gall

K Sumikawa

G Lynch

Affiliations

Soon will be listed here.

Abstract

In situ hybridization and RNA blot-hybridization techniques were used (i) to examine the regional distribution of mRNA for a putative kainate receptor in adult rat brain and ii) to test the possibility that seizures affect expression of the receptor gene. The highest densities of hybridization were distributed within hippocampal pyramidal and granule cells, medial habenula, Purkinje cells and the molecular layer of cerebellum, and olfactory bulb. Recurrent limbic seizures caused a massive, delayed, and reversible reduction in levels of the kainate receptor mRNA in dentate gyrus; lesser decreases were found in pyramidal cell fields of hippocampus and superficial cortex. These findings provide evidence that unusual patterns of physiological activity can alter genomic expression for a subclass of glutamate receptors in brain.

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References

Unnerstall J, Wamsley J . Autoradiographic localization of high-affinity [3H]kainic acid binding sites in the rat forebrain. Eur J Pharmacol. 1983; 86(3-4):361-71. DOI: 10.1016/0014-2999(83)90185-1. View

Monaghan D, Cotman C . The distribution of [3H]kainic acid binding sites in rat CNS as determined by autoradiography. Brain Res. 1982; 252(1):91-100. DOI: 10.1016/0006-8993(82)90981-7. View

Campbell K, Bank B, Milgram N . Epileptogenic effects of electrolytic lesions in the hippocampus: role of iron deposition. Exp Neurol. 1984; 86(3):506-14. DOI: 10.1016/0014-4886(84)90085-2. View

Monaghan D, Yao D, Cotman C . L-[3H]Glutamate binds to kainate-, NMDA- and AMPA-sensitive binding sites: an autoradiographic analysis. Brain Res. 1985; 340(2):378-83. DOI: 10.1016/0006-8993(85)90936-9. View

Heimer L, Zaborszky L, Zahm D, Alheid G . The ventral striatopallidothalamic projection: I. The striatopallidal link originating in the striatal parts of the olfactory tubercle. J Comp Neurol. 1987; 255(4):571-91. DOI: 10.1002/cne.902550409. View

White J, Gall C, McKelvy J . Enkephalin biosynthesis and enkephalin gene expression are increased in hippocampal mossy fibers following a unilateral lesion of the hilus. J Neurosci. 1987; 7(3):753-9. PMC: 6569070. View

Chomczynski P, Sacchi N . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162(1):156-9. DOI: 10.1006/abio.1987.9999. View

White J, Gall C . Differential regulation of neuropeptide and proto-oncogene mRNA content in the hippocampus following recurrent seizures. Brain Res. 1987; 427(1):21-9. DOI: 10.1016/0169-328x(87)90040-4. View

Sumikawa K, Miledi R . Repression of nicotinic acetylcholine receptor expression by antisense RNAs and an oligonucleotide. Proc Natl Acad Sci U S A. 1988; 85(4):1302-6. PMC: 279755. DOI: 10.1073/pnas.85.4.1302. View

10.

Saiki R, Gelfand D, Stoffel S, Scharf S, Higuchi R, Horn G . Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988; 239(4839):487-91. DOI: 10.1126/science.2448875. View

11.

Gall C . Seizures induce dramatic and distinctly different changes in enkephalin, dynorphin, and CCK immunoreactivities in mouse hippocampal mossy fibers. J Neurosci. 1988; 8(6):1852-62. PMC: 6569340. View

12.

Brehm P, Henderson L . Regulation of acetylcholine receptor channel function during development of skeletal muscle. Dev Biol. 1988; 129(1):1-11. DOI: 10.1016/0012-1606(88)90156-x. View

13.

Saffen D, Cole A, Worley P, Christy B, Ryder K, Baraban J . Convulsant-induced increase in transcription factor messenger RNAs in rat brain. Proc Natl Acad Sci U S A. 1988; 85(20):7795-9. PMC: 282280. DOI: 10.1073/pnas.85.20.7795. View

14.

Gall C, Isackson P . Limbic seizures increase neuronal production of messenger RNA for nerve growth factor. Science. 1989; 245(4919):758-61. DOI: 10.1126/science.2549634. View

15.

Pico R, Gall C . Continuities between outer nuclear membrane and the rough endoplasmic reticulum increase in hippocampal neurons during seizure-induced protein synthesis. Brain Res. 1989; 497(2):387-92. DOI: 10.1016/0006-8993(89)90286-2. View

16.

Hollmann M, ROGERS S, Heinemann S . Cloning by functional expression of a member of the glutamate receptor family. Nature. 1989; 342(6250):643-8. DOI: 10.1038/342643a0. View

17.

Racine R . Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol. 1972; 32(3):281-94. DOI: 10.1016/0013-4694(72)90177-0. View

18.

Graybiel A, Ragsdale Jr C . Histochemically distinct compartments in the striatum of human, monkeys, and cat demonstrated by acetylthiocholinesterase staining. Proc Natl Acad Sci U S A. 1978; 75(11):5723-6. PMC: 393041. DOI: 10.1073/pnas.75.11.5723. View

19.

Macrides F, Eichenbaum H, Forbes W . Temporal relationship between sniffing and the limbic theta rhythm during odor discrimination reversal learning. J Neurosci. 1982; 2(12):1705-17. PMC: 6564372. View

20.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View