Occupational Exposure to Diisononyl Phthalate (DiNP) in Polyvinyl Chloride Processing Operations

Overview

Journal Int Arch Occup Environ Health

Specialty Environmental Health

Date 2011 Jun 25

PMID 21701833

Citations 24

Authors

Cynthia J Hines

Nancy B Hopf

James A Deddens

Manori J Silva

Antonia M Calafat

Affiliations

Soon will be listed here.

Abstract

Purpose: Diisononyl phthalate (DiNP) is primarily used as a plasticizer in polyvinyl chloride (PVC) materials. While information is available on general population exposure to DiNP, occupational exposure data are lacking. We present DiNP metabolite urinary concentrations in PVC processing workers, estimate DiNP daily intake for these workers, and compare worker estimates to other populations.

Methods: We assessed DiNP exposure in participants from two companies that manufactured PVC materials, a PVC film manufacturer (n = 25) and a PVC custom compounder (n = 12). A mid-shift and end-shift urine sample was collected from each participant and analyzed for the DiNP metabolite mono(carboxy-isooctyl) phthalate (MCiOP). Mixed models were used to assess the effect on MCiOP concentrations of a worker being assigned to (1) a task using DiNP and (2) a shift where DiNP was used. A simple pharmacokinetic model was used to estimate DiNP daily intake from the MCiOP concentrations.

Results: Creatinine-adjusted MCiOP urinary concentrations ranged from 0.42-80 μg/g in PVC film and from 1.11-13.4 μg/g in PVC compounding. PVC film participants who worked on a task using DiNP (n = 7) had the highest MCiOP geometric mean (GM) end-shift concentration (25.2 μg/g), followed by participants who worked on a shift where DiNP was used (n = 11) (17.7 μg/g) as compared to participants with no task (2.92 μg/g) or shift (2.08 μg/g) exposure to DiNP. The GM end-shift MCiOP concentration in PVC compounding participants (4.80 μg/g) was comparable to PVC film participants with no task or shift exposure to DiNP. Because no PVC compounding participants were assigned to tasks using DINP on the day sampled, DiNP exposure in this company may be underestimated. The highest DiNP intake estimate was 26 μg/kg/day.

Conclusion: Occupational exposure to DiNP associated with PVC film manufacturing tasks were substantially higher (sixfold to tenfold) than adult general population exposures; however, all daily intake estimates were less than 25% of current United States or European acceptable or tolerable daily intake estimates. Further characterization of DiNP occupational exposures in other industries is recommended.

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References

Calafat A, Wong L, Silva M, Samandar E, Preau Jr J, Jia L . Selecting adequate exposure biomarkers of diisononyl and diisodecyl phthalates: data from the 2005-2006 National Health and Nutrition Examination Survey. Environ Health Perspect. 2010; 119(1):50-5. PMC: 3018499. DOI: 10.1289/ehp.1002316. View

Silva M, Kato K, Wolf C, Samandar E, Silva S, Gray E . Urinary biomarkers of di-isononyl phthalate in rats. Toxicology. 2006; 223(1-2):101-12. DOI: 10.1016/j.tox.2006.03.005. View

Adamsson A, Salonen V, Paranko J, Toppari J . Effects of maternal exposure to di-isononylphthalate (DINP) and 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (p,p'-DDE) on steroidogenesis in the fetal rat testis and adrenal gland. Reprod Toxicol. 2009; 28(1):66-74. DOI: 10.1016/j.reprotox.2009.03.002. View

Waterman S, Ambroso J, Keller L, Trimmer G, Nikiforov A, Harris S . Developmental toxicity of di-isodecyl and di-isononyl phthalates in rats. Reprod Toxicol. 1999; 13(2):131-6. DOI: 10.1016/s0890-6238(99)00002-7. View

Lington A, Bird M, Plutnick R, Stubblefield W, Scala R . Chronic toxicity and carcinogenic evaluation of diisononyl phthalate in rats. Fundam Appl Toxicol. 1997; 36(1):79-89. DOI: 10.1006/faat.1997.2286. View

Kaufmann W, Deckardt K, McKee R, Butala J, Bahnemann R . Tumor induction in mouse liver: di-isononyl phthalate acts via peroxisome proliferation. Regul Toxicol Pharmacol. 2002; 36(2):175-83. DOI: 10.1006/rtph.2002.1575. View

Scott R, Dugard P, Ramsey J, Rhodes C . In vitro absorption of some o-phthalate diesters through human and rat skin. Environ Health Perspect. 1987; 74:223-7. PMC: 1474516. DOI: 10.1289/ehp.8774223. View

Klaunig J, Babich M, Baetcke K, Cook J, Corton J, David R . PPARalpha agonist-induced rodent tumors: modes of action and human relevance. Crit Rev Toxicol. 2004; 33(6):655-780. DOI: 10.1080/713608372. View

Blount B, Silva M, Caudill S, Needham L, Pirkle J, Sampson E . Levels of seven urinary phthalate metabolites in a human reference population. Environ Health Perspect. 2000; 108(10):979-82. PMC: 1240132. DOI: 10.1289/ehp.00108979. View

10.

Boberg J, Christiansen S, Axelstad M, Kledal T, Vinggaard A, Dalgaard M . Reproductive and behavioral effects of diisononyl phthalate (DINP) in perinatally exposed rats. Reprod Toxicol. 2010; 31(2):200-9. DOI: 10.1016/j.reprotox.2010.11.001. View

11.

Wormuth M, Scheringer M, Vollenweider M, Hungerbuhler K . What are the sources of exposure to eight frequently used phthalic acid esters in Europeans?. Risk Anal. 2006; 26(3):803-24. DOI: 10.1111/j.1539-6924.2006.00770.x. View

12.

Ye X, Pierik F, Hauser R, Duty S, Angerer J, Park M . Urinary metabolite concentrations of organophosphorous pesticides, bisphenol A, and phthalates among pregnant women in Rotterdam, the Netherlands: the Generation R study. Environ Res. 2008; 108(2):260-7. PMC: 2628162. DOI: 10.1016/j.envres.2008.07.014. View

13.

Silva M, Reidy J, Preau Jr J, Needham L, Calafat A . Oxidative metabolites of diisononyl phthalate as biomarkers for human exposure assessment. Environ Health Perspect. 2006; 114(8):1158-61. PMC: 1552017. DOI: 10.1289/ehp.8865. View

14.

Garrod A, Phillips A, Pemberton J . Potential exposure of hands inside protective gloves-a summary of data from non-agricultural pesticide surveys. Ann Occup Hyg. 2001; 45(1):55-60. View

15.

Pugh Jr G, Isenberg J, Kamendulis L, Ackley D, Clare L, Brown R . Effects of di-isononyl phthalate, di-2-ethylhexyl phthalate, and clofibrate in cynomolgus monkeys. Toxicol Sci. 2000; 56(1):181-8. DOI: 10.1093/toxsci/56.1.181. View

16.

Koch H, Calafat A . Human body burdens of chemicals used in plastic manufacture. Philos Trans R Soc Lond B Biol Sci. 2009; 364(1526):2063-78. PMC: 2873011. DOI: 10.1098/rstb.2008.0208. View

17.

Hines C, Deddens J, Tucker S, Hornung R . Distributions and determinants of pre-emergent herbicide exposures among custom applicators. Ann Occup Hyg. 2001; 45(3):227-39. DOI: 10.1093/annhyg/45.3.227. View

18.

Kohn M, Parham F, Masten S, Portier C, Shelby M, Brock J . Human exposure estimates for phthalates. Environ Health Perspect. 2000; 108(10):A440-2. PMC: 1240144. DOI: 10.1289/ehp.108-a440b. View

19.

Latini G, Wittassek M, Del Vecchio A, Presta G, De Felice C, Angerer J . Lactational exposure to phthalates in Southern Italy. Environ Int. 2008; 35(2):236-9. DOI: 10.1016/j.envint.2008.06.002. View

20.

Koch H, Muller J, Angerer J . Determination of secondary, oxidised di-iso-nonylphthalate (DINP) metabolites in human urine representative for the exposure to commercial DINP plasticizers. J Chromatogr B Analyt Technol Biomed Life Sci. 2006; 847(2):114-25. DOI: 10.1016/j.jchromb.2006.09.044. View