Down-regulated MiRNA-214 Induces a Cell Cycle G1 Arrest in Gastric Cancer Cells by Up-regulating the PTEN Protein

Overview

Journal Pathol Oncol Res

Specialty Oncology

Date 2011 Jun 21

PMID 21688200

Citations 31

Authors

Xin Xiong

Hong-Zheng Ren

Min-Hua Li

Jin-Hong Mei

Ji-Fang Wen

Chang-Li Zheng

Affiliations

Soon will be listed here.

Abstract

To detect the expression of miRNA-214 in human gastric cancer cell lines of BGC823, MKN45 and SGC7901, and to identify the effect of miRNA-214 on cell cycle and apoptosis of these cells. Expression of miRNA-214 in human normal gastric mucosal cell line GES-1 and human gastric cancer cell lines was detected by real-time reverse-transcription polymerase chain reaction. Antisense-miRNA-214 oligonucleotides were transfected transiently into gastric cancer cell lines to down-regulate the expression of miRNA-214. The cell cycle and apoptosis were studied by flow cytometry assay. PTEN, one of the target genes of miRNA-214 was detected by using of immunocytochemistry and Western blotting. MiRNA-214 was overexpressed in gastric cancer cell lines of BGC823, MKN45 and SGC7901 compared with normal gastric mucosal cell line GES-1. Antisense-miRNA-214 oligonucleotides significantly down-regulated the expression of miRNA-214, and increased the portion of G1-phase and decreased the portion of S-phase in BGC823 and MKN45 cells. The immunocytochemistry test and Western blotting analysis showed that the down-regulation of miRNA-214 could significantly up-regulate the expression of PTEN in BGC823 and MKN45 cells. MiRNA-214 is overexpressed in human gastric cancer cell lines of BGC823, MKN45 and SGC7901. The down-regulation of miRNA-214 could induce a G1 cell cycle arrest in them, the up-regulation of PTEN maybe one of the mechanism.

Citing Articles

The Role of Autophagy in Human Uveal Melanoma and the Development of Potential Disease Biomarkers and Novel Therapeutic Paradigms.

Wang J, Paulus P, Niu Y, Zhu L, Morisseau C, Rawling T Biomedicines. 2024; 12(2.

PMID: 38398064 PMC: 10886749. DOI: 10.3390/biomedicines12020462.

Study of miRNA and lymphocyte subsets as potential biomarkers for the diagnosis and prognosis of gastric cancer.

Li J, Chen Z, Li Q, Liu R, Zheng J, Gu Q PeerJ. 2024; 12:e16660.

PMID: 38259671 PMC: 10802158. DOI: 10.7717/peerj.16660.

Potential Diagnostic Utility of microRNAs in Gastrointestinal Cancers.

Jelski W, Mroczko B Cancer Manag Res. 2023; 15:863-871.

PMID: 37636029 PMC: 10460163. DOI: 10.2147/CMAR.S421928.

Molecular and Circulating Biomarkers of Gastric Cancer.

Jelski W, Mroczko B Int J Mol Sci. 2022; 23(14).

PMID: 35886934 PMC: 9322632. DOI: 10.3390/ijms23147588.

Pro-Apoptotic Activity of the Marine Sponge Metabolites Pelorol and 5--Ilimaquinone on Human 501Mel Melanoma Cells.

Carpi S, Scoditti E, Polini B, Brogi S, Calderone V, Proksch P Mar Drugs. 2022; 20(7).

PMID: 35877720 PMC: 9317990. DOI: 10.3390/md20070427.

References

Cimmino A, Calin G, Fabbri M, Iorio M, Ferracin M, Shimizu M . miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci U S A. 2005; 102(39):13944-9. PMC: 1236577. DOI: 10.1073/pnas.0506654102. View

Eis P, Tam W, Sun L, Chadburn A, Li Z, Gomez M . Accumulation of miR-155 and BIC RNA in human B cell lymphomas. Proc Natl Acad Sci U S A. 2005; 102(10):3627-32. PMC: 552785. DOI: 10.1073/pnas.0500613102. View

Volinia S, Calin G, Liu C, Ambs S, Cimmino A, Petrocca F . A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A. 2006; 103(7):2257-61. PMC: 1413718. DOI: 10.1073/pnas.0510565103. View

Chen C . MicroRNAs as oncogenes and tumor suppressors. N Engl J Med. 2005; 353(17):1768-71. DOI: 10.1056/NEJMp058190. View

Decembrini S, Bressan D, Vignali R, Pitto L, Mariotti S, Rainaldi G . MicroRNAs couple cell fate and developmental timing in retina. Proc Natl Acad Sci U S A. 2009; 106(50):21179-84. PMC: 2781736. DOI: 10.1073/pnas.0909167106. View

Flynt A, Li N, Thatcher E, Solnica-Krezel L, Patton J . Zebrafish miR-214 modulates Hedgehog signaling to specify muscle cell fate. Nat Genet. 2007; 39(2):259-63. PMC: 3982799. DOI: 10.1038/ng1953. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Zhang H, Li M, Han Y, Hong L, Gong T, Sun L . Down-regulation of miR-27a might reverse multidrug resistance of esophageal squamous cell carcinoma. Dig Dis Sci. 2009; 55(9):2545-51. DOI: 10.1007/s10620-009-1051-6. View

Chan J, Krichevsky A, Kosik K . MicroRNA-21 is an antiapoptotic factor in human glioblastoma cells. Cancer Res. 2005; 65(14):6029-33. DOI: 10.1158/0008-5472.CAN-05-0137. View

10.

Papagiannakopoulos T, Shapiro A, Kosik K . MicroRNA-21 targets a network of key tumor-suppressive pathways in glioblastoma cells. Cancer Res. 2008; 68(19):8164-72. DOI: 10.1158/0008-5472.CAN-08-1305. View

11.

Xia L, Zhang D, Du R, Pan Y, Zhao L, Sun S . miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells. Int J Cancer. 2008; 123(2):372-379. DOI: 10.1002/ijc.23501. View

12.

Yang H, Kong W, He L, Zhao J, ODonnell J, Wang J . MicroRNA expression profiling in human ovarian cancer: miR-214 induces cell survival and cisplatin resistance by targeting PTEN. Cancer Res. 2008; 68(2):425-33. DOI: 10.1158/0008-5472.CAN-07-2488. View

13.

Zhang B, Pan X, Cobb G, Anderson T . microRNAs as oncogenes and tumor suppressors. Dev Biol. 2006; 302(1):1-12. DOI: 10.1016/j.ydbio.2006.08.028. View

14.

Motoyama K, Inoue H, Nakamura Y, Uetake H, Sugihara K, Mori M . Clinical significance of high mobility group A2 in human gastric cancer and its relationship to let-7 microRNA family. Clin Cancer Res. 2008; 14(8):2334-40. DOI: 10.1158/1078-0432.CCR-07-4667. View

15.

Fei J, Lan F, Guo M, Li Y, Liu Y . Inhibitory effects of anti-miRNA oligonucleotides (AMOs) on A549 cell growth. J Drug Target. 2008; 16(9):688-93. DOI: 10.1080/10611860802295946. View

16.

Petrocca F, Visone R, Onelli M, Shah M, Nicoloso M, De Martino I . E2F1-regulated microRNAs impair TGFbeta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell. 2008; 13(3):272-86. DOI: 10.1016/j.ccr.2008.02.013. View

17.

Kluiver J, Haralambieva E, de Jong D, Blokzijl T, Jacobs S, Kroesen B . Lack of BIC and microRNA miR-155 expression in primary cases of Burkitt lymphoma. Genes Chromosomes Cancer. 2005; 45(2):147-53. DOI: 10.1002/gcc.20273. View

18.

Ihara M, Yamamoto H, Kikuchi A . SUMO-1 modification of PIASy, an E3 ligase, is necessary for PIASy-dependent activation of Tcf-4. Mol Cell Biol. 2005; 25(9):3506-18. PMC: 1084305. DOI: 10.1128/MCB.25.9.3506-3518.2005. View

19.

Chan S, Wu C, Li A, Chi C, Lin W . miR-21 microRNA expression in human gastric carcinomas and its clinical association. Anticancer Res. 2008; 28(2A):907-11. View

20.

Kim D, Chae H, Oh S, Kang J, Park C, Park W . Expression of viral microRNAs in Epstein-Barr virus-associated gastric carcinoma. J Virol. 2006; 81(2):1033-6. PMC: 1797424. DOI: 10.1128/JVI.02271-06. View