Isolation and Characterization of a Novel Alphanodavirus

Overview

Journal Virol J

Publisher Biomed Central

Specialty Microbiology

Date 2011 Jun 21

PMID 21682922

Citations 4

Authors

Huimin Bai

Yun Wang

Xiang Li

Haitao Mao

Yan Li

Shili Han

Zhengli Shi

Xinwen Chen

Affiliations

Soon will be listed here.

Abstract

Background: Nodaviridae is a family of non-enveloped isometric viruses with bipartite positive-sense RNA genomes. The Nodaviridae family consists of two genera: alpha- and beta-nodavirus. Alphanodaviruses usually infect insect cells. Some commercially available insect cell lines have been latently infected by Alphanodaviruses.

Results: A non-enveloped small virus of approximately 30 nm in diameter was discovered co-existing with a recombinant Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) in Hz-AM1 cells. Genome sequencing and phylogenetic assays indicate that this novel virus belongs to the genus of alphanodavirus in the family Nodaviridae and was designated HzNV. HzNV possesses a RNA genome that contains two segments. RNA1 is 3038 nt long and encodes a 110 kDa viral protein termed protein A. The 1404 nt long RNA2 encodes a 44 kDa protein, which exhibits a high homology with coat protein precursors of other alphanodaviruses. HzNV virions were located in the cytoplasm, in association with cytoplasmic membrane structures. The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells. However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.

Conclusion: A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.

Citing Articles

The taxonomy of an Australian nodavirus isolated from mosquitoes.

Warrilow D, Huang B, Newton N, Harrison J, Johnson K, Chow W PLoS One. 2019; 13(12):e0210029.

PMID: 30596795 PMC: 6312370. DOI: 10.1371/journal.pone.0210029.

Adventitious viruses in insect cell lines used for recombinant protein expression.

Geisler C, Jarvis D Protein Expr Purif. 2017; 144:25-32.

PMID: 29133148 PMC: 5826799. DOI: 10.1016/j.pep.2017.11.002.

A unique nodavirus with novel features: mosinovirus expresses two subgenomic RNAs, a capsid gene of unknown origin, and a suppressor of the antiviral RNA interference pathway.

Schuster S, Zirkel F, Kurth A, van Cleef K, Drosten C, van Rij R J Virol. 2014; 88(22):13447-59.

PMID: 25210176 PMC: 4249075. DOI: 10.1128/JVI.02144-14.

Next generation sequencing technologies for insect virus discovery.

Liu S, Vijayendran D, Bonning B Viruses. 2011; 3(10):1849-69.

PMID: 22069519 PMC: 3205385. DOI: 10.3390/v3101849.

References

Friesen P, Rueckert R . Black beetle virus: messenger for protein B is a subgenomic viral RNA. J Virol. 1982; 42(3):986-95. PMC: 256932. DOI: 10.1128/JVI.42.3.986-995.1982. View

Pijlman G, Pruijssers A, Vlak J . Identification of pif-2, a third conserved baculovirus gene required for per os infection of insects. J Gen Virol. 2003; 84(Pt 8):2041-2049. DOI: 10.1099/vir.0.19133-0. View

Hatta T, Bullivant S, Matthews R . Fine structure of vesicles induced in chloroplasts of Chinese cabbage leaves by infection with turnip yellow mosaic virus. J Gen Virol. 1973; 20(1):37-50. DOI: 10.1099/0022-1317-20-1-37. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Schneemann A, Reddy V, Johnson J . The structure and function of nodavirus particles: a paradigm for understanding chemical biology. Adv Virus Res. 1998; 50:381-446. DOI: 10.1016/s0065-3527(08)60812-x. View

Li T, Scotti P, Miyamura T, Takeda N . Latent infection of a new alphanodavirus in an insect cell line. J Virol. 2007; 81(20):10890-6. PMC: 2045576. DOI: 10.1128/JVI.00807-07. View

Froshauer S, Kartenbeck J, Helenius A . Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes. J Cell Biol. 1988; 107(6 Pt 1):2075-86. PMC: 2115628. DOI: 10.1083/jcb.107.6.2075. View

Tamura K, Dudley J, Nei M, Kumar S . MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007; 24(8):1596-9. DOI: 10.1093/molbev/msm092. View

Vaughn J, GOODWIN R, Tompkins G, McCawley P . The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro. 1977; 13(4):213-7. DOI: 10.1007/BF02615077. View

10.

Miller D, Schwartz M, Ahlquist P . Flock house virus RNA replicates on outer mitochondrial membranes in Drosophila cells. J Virol. 2001; 75(23):11664-76. PMC: 114753. DOI: 10.1128/JVI.75.23.11664-11676.2001. View

11.

Guo Y, Chan S, Kwang J . Membrane association of greasy grouper nervous necrosis virus protein A and characterization of its mitochondrial localization targeting signal. J Virol. 2004; 78(12):6498-508. PMC: 416515. DOI: 10.1128/JVI.78.12.6498-6508.2004. View

12.

Pedersen K, van der Meer Y, Roos N, Snijder E . Open reading frame 1a-encoded subunits of the arterivirus replicase induce endoplasmic reticulum-derived double-membrane vesicles which carry the viral replication complex. J Virol. 1999; 73(3):2016-26. PMC: 104444. DOI: 10.1128/JVI.73.3.2016-2026.1999. View

13.

Bailey L, NEWMAN J, PORTERFIELD J . The multiplication of Nodamura virus in insect and mammalian cell cultures. J Gen Virol. 1975; 26(1):15-20. DOI: 10.1099/0022-1317-26-1-15. View

14.

Weber-Lotfi F, Dietrich A, Russo M, Rubino L . Mitochondrial targeting and membrane anchoring of a viral replicase in plant and yeast cells. J Virol. 2002; 76(20):10485-96. PMC: 136569. DOI: 10.1128/jvi.76.20.10485-10496.2002. View

15.

Gallagher T, Rueckert R . Assembly-dependent maturation cleavage in provirions of a small icosahedral insect ribovirus. J Virol. 1988; 62(9):3399-406. PMC: 253463. DOI: 10.1128/JVI.62.9.3399-3406.1988. View

16.

Widada J, Durand S, Cambournac I, Qian D, Shi Z, Dejonghe E . Genome-based detection methods of Macrobrachium rosenbergii nodavirus, a pathogen of the giant freshwater prawn, Macrobrachium rosenbergii dot-blot, in situ hybridization and RT-PCR. J Fish Dis. 2003; 26(10):583-90. DOI: 10.1046/j.1365-2761.2003.00493.x. View

17.

Johnson K, Johnson K, Dasgupta R, Gratsch T, Ball L . Comparisons among the larger genome segments of six nodaviruses and their encoded RNA replicases. J Gen Virol. 2001; 82(Pt 8):1855-1866. DOI: 10.1099/0022-1317-82-8-1855. View

18.

Koonin E . The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. J Gen Virol. 1991; 72 ( Pt 9):2197-206. DOI: 10.1099/0022-1317-72-9-2197. View

19.

Garzon S, Strykowski H, Charpentier G . Implication of mitochondria in the replication of Nodamura virus in larvae of the Lepidoptera, Galleria mellonella (L.) and in suckling mice. Arch Virol. 1990; 113(3-4):165-76. DOI: 10.1007/BF01316670. View

20.

Li H, Li W, Ding S . Induction and suppression of RNA silencing by an animal virus. Science. 2002; 296(5571):1319-21. DOI: 10.1126/science.1070948. View