BiP and Multiple DNAJ Molecular Chaperones in the Endoplasmic Reticulum Are Required for Efficient Simian Virus 40 Infection

Overview

Journal mBio

Publisher American Society for Microbiology

Specialty Microbiology

Date 2011 Jun 16

PMID 21673190

Citations 69

Authors

Edward C Goodwin

Alex Lipovsky

Takamasa Inoue

Thomas G Magaldi

Anne P B Edwards

Kristin E Y Van Goor

Adrienne W Paton

James C Paton

Walter J Atwood

Billy Tsai

Daniel DiMaio

Affiliations

Soon will be listed here.

Abstract

Simian virus 40 (SV40) is a nonenveloped DNA virus that traffics through the endoplasmic reticulum (ER) en route to the nucleus, but the mechanisms of capsid disassembly and ER exit are poorly understood. We conducted an unbiased RNA interference screen to identify cellular genes required for SV40 infection. SV40 infection was specifically inhibited by up to 50-fold by knockdown of four different DNAJ molecular cochaperones or by inhibition of BiP, the Hsp70 partner of DNAJB11. These proteins were not required for the initiation of capsid disassembly, but knockdown markedly inhibited SV40 exit from the ER. In addition, BiP formed a complex with SV40 capsids in the ER in a DNAJB11-dependent fashion. These experiments identify five new cellular proteins required for SV40 infection and suggest that the binding of BiP to the capsid is required for ER exit. Further studies of these proteins will provide insight into the molecular mechanisms of polyomavirus infection and ER function.

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