Mouse Mammary Tumor Virus (MMTV) Infection in SWISS and RIII Mice. Correlation Between Resistance to Exogenous Infection and Anti-MMTV Serum Response

Overview

Journal Arch Virol

Specialty Microbiology

Date 1990 Jan 1

PMID 2167058

Authors

P Hainaut

D Vaira

C Francois

C M Calberg-Bacq

Affiliations

Soon will be listed here.

Abstract

Host-virus relationships were examined in mice from the two mouse mammary tumor virus (MMTV)-infected strains SWISS MB+ and RIII, which harbour the same MMTV variant, and from the derived sublines Swiss MB- and RIIIf, which were freed of milk-borne MMTV by foster-nursing. These two strains are not phylogenetically related, the SWISS strain bearing the endogenous Mtv-3 locus in its DNA. In RIII and SWISS MB+ mice, the incidence of early mammary tumors, which was of 96% and 8%, respectively, was correlated to the level of MMTV expression in milk. In the SWISS MB-line, a non-coordinate expression of the provirus associated with the Mtv-3 locus was observed in the mammary glands, the salivary glands and the spleen. This expression was not tumorigenic and was characterized by the presence of the p28 gag antigen and the absence of the gp52 env antigen, except, however, in mammary glands of elder mice where traces of gp52 were found. In the mammary glands of SWISS MB+ mice, the expression of the Mtv-3 locus was masked by large amounts of antigens resulting from exogenous virus expression. RIIIf mice were MMTV-negative. Viral antigens coexisted with anti-MMTV antibodies in the serum of infected and tumor-bearing mice, but not in the form of immune complexes as verified by a method that allowed to detect specific antigen-containing-soluble immune complexes. An anti-MMTV serum reactivity was also detected in SWISS MB- and RIIIf mice. However, the serum response was higher in the two SWISS lines than in the two RIII lines. Except in tumor-bearing mice, the anti-MMTV response was not significantly modified by the presence of exogenous virus and thus resulted essentially from exposure to endogenous MMTV expression. In experimental infection studies, RIII mice were more susceptible to MMTV infection than SWISS mice. The correlation between resistance to MMTV infection and serum response to endogenous MMTV expression, suggests that the non-tumorigenic expression of an endogenous provirus can protect at least partially, against exogenous MMTV infection.

References

Nusse R, Varmus H . Many tumors induced by the mouse mammary tumor virus contain a provirus integrated in the same region of the host genome. Cell. 1982; 31(1):99-109. DOI: 10.1016/0092-8674(82)90409-3. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Buetti E, DIGGELMANN H . Cloned mouse mammary tumor virus DNA is biologically active in transfected mouse cells and its expression is stimulated by glucocorticoid hormones. Cell. 1981; 23(2):335-45. DOI: 10.1016/0092-8674(81)90129-x. View

Altrock B, Cardiff R, Blair P . Murine mammary tumor virus seroepidemiology in BALB/cfC3H mice: correlation with tumor development. J Natl Cancer Inst. 1981; 67(1):163-8. View

Ihle J, Arthur L, Fine D . Autogenous immunity to mouse mammary tumor virus in mouse strains of high and low mammary tumor incidence. Cancer Res. 1976; 36(8):2840-4. View

Michalides R, Wagenaar E, Weijers P . Rearrangements in the long terminal repeat of extra mouse mammary tumor proviruses in T-cell leukemias of mouse strain GR result in a novel enhancer-like structure. Mol Cell Biol. 1985; 5(4):823-30. PMC: 366787. DOI: 10.1128/mcb.5.4.823-830.1985. View

VAN NIE R, Verstraeten A, de Moes J . Genetic transmission of mammary tumour virus by GR mice. Int J Cancer. 1977; 19(3):383-90. DOI: 10.1002/ijc.2910190316. View

Michalides R, Verstraeten R, Shen F, Hilgers J . Characterization and chromosomal distribution of endogenous mouse mammary tumor viruses of European mouse strains STS/A and GR/A. Virology. 1985; 142(2):278-90. DOI: 10.1016/0042-6822(85)90336-8. View

Ritzi E, Martin D, Stolfi R, Spiegelman S . Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model. Proc Natl Acad Sci U S A. 1976; 73(11):4190-4. PMC: 431381. DOI: 10.1073/pnas.73.11.4190. View

10.

VAN NIE R, Verstraeten A . Studies of genetic transmission of mammary tumour virus by C3Hf mice. Int J Cancer. 1975; 16(6):922-31. DOI: 10.1002/ijc.2910160606. View

11.

Nusse R, de Moes J, Hilkens J, VAN NIE R . Localization of a gene for expression of mouse mammary tumor virus antigens in the GR/Mtv-2- mouse strain. J Exp Med. 1980; 152(3):712-9. PMC: 2185924. DOI: 10.1084/jem.152.3.712. View

12.

HILGERS J, Theuns G, VAN NIE R . Mammary tumor virus (MTV) antigens in normal and mammary tumor-bearing mice. Int J Cancer. 1973; 12(3):568-76. DOI: 10.1002/ijc.2910120305. View

13.

Robinson H, Astrin S, Senior A, Salazar F . Host Susceptibility to endogenous viruses: defective, glycoprotein-expressing proviruses interfere with infections. J Virol. 1981; 40(3):745-51. PMC: 256686. DOI: 10.1128/JVI.40.3.745-751.1981. View

14.

Nusse R, van der Ploeg L, van Duijn L, Michalides R, Hilgers J . Impaired maturation of mouse mammary tumor virus precursor polypeptides in lymphoid leukemia cells, producing intracytoplasmic A particles and no extracellular B-type virions. J Virol. 1979; 32(1):251-8. PMC: 353549. DOI: 10.1128/JVI.32.1.251-258.1979. View

15.

STECK F, Rubin H . The mechanism of interference between an avian leukosis virus and Rous sarcoma virus. II. Early steps of infection by RSV of cells under conditions of interference. Virology. 1966; 29(4):642-53. DOI: 10.1016/0042-6822(66)90288-1. View

16.

Noon M, WOLFORD R, Parks W . Expression of mouse mammary tumor viral polypeptides in milks and tissues. J Immunol. 1975; 115(3):653-8. View

17.

Peters G, Brookes S, Smith R, Dickson C . Tumorigenesis by mouse mammary tumor virus: evidence for a common region for provirus integration in mammary tumors. Cell. 1983; 33(2):369-77. DOI: 10.1016/0092-8674(83)90418-x. View

18.

Zotter S, Lossnitzer A, Klahr G . Mammary tumour virus (MTV)-specific immune complex deposites in renal glomeruli of MTV-infected tumour-free mice. Acta Biol Med Ger. 1976; 35(2):253-7. View

19.

OSTERRIETH P, Kozma S, Hendrick J, Francois C, Calberg-Bacq C, Franchimont P . Detection of virus antigens in Swiss albino mice infected by milk-borne mouse mammary tumour virus: the effect of age, sex and reproductive status. II. Radioimmunoassay of two virus components, gp47 and p28 in serum and organ extracts. J Gen Virol. 1979; 45(1):41-50. DOI: 10.1099/0022-1317-45-1-41. View

20.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View