Calcium-dependent Isoforms of Protein Kinase C Mediate Posttetanic Potentiation at the Calyx of Held

Overview

Journal Neuron

Publisher Cell Press

Specialty Neurology

Date 2011 Jun 11

PMID 21658591

Citations 36

Authors

Diasynou Fioravante

Yunxiang Chu

Michael H Myoga

Michael Leitges

Wade G Regehr

Affiliations

Soon will be listed here.

Abstract

High-frequency stimulation leads to a transient increase in the amplitude of evoked synaptic transmission that is known as posttetanic potentiation (PTP). Here we examine the roles of the calcium-dependent protein kinase C isoforms PKCα and PKCβ in PTP at the calyx of Held synapse. In PKCα/β double knockouts, 80% of PTP is eliminated, whereas basal synaptic properties are unaffected. PKCα and PKCβ produce PTP by increasing the size of the readily releasable pool of vesicles evoked by high-frequency stimulation and by increasing the fraction of this pool released by the first stimulus. PKCα and PKCβ do not facilitate presynaptic calcium currents. The small PTP remaining in double knockouts is mediated partly by an increase in miniature excitatory postsynaptic current amplitude and partly by a mechanism involving myosin light chain kinase. These experiments establish that PKCα and PKCβ are crucial for PTP and suggest that long-lasting presynaptic calcium increases produced by tetanic stimulation may activate these isoforms to produce PTP.

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