A Xylosylphosphotransferase of Cryptococcus Neoformans Acts in Protein O-glycan Synthesis

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2011 May 25

PMID 21606487

Citations 15

Authors

Morgann C Reilly

Kazuhiro Aoki

Zhuo A Wang

Michael L Skowyra

Matthew Williams

Michael Tiemeyer

Tamara L Doering

Affiliations

Soon will be listed here.

Abstract

Cryptococcal meningoencephalitis is an AIDS-defining illness caused by the opportunistic pathogen Cryptococcus neoformans. This organism possesses an elaborate polysaccharide capsule that is unique among pathogenic fungi, and the glycobiology of C. neoformans has been a focus of research in the field. The capsule and other cellular glycans and glycoconjugates have been described, but the machinery responsible for their synthesis remains largely unexplored. We recently discovered Xpt1p, an enzyme with the unexpected activity of generating a xylose-phosphate-mannose linkage. We now demonstrate that this novel activity is conserved throughout the C. neoformans species complex, localized to the Golgi apparatus, and functions in the O-glycosylation of proteins. We also present the first survey of O-glycans from C. neoformans.

Citing Articles

Extension of -Linked Mannosylation in the Golgi Apparatus Is Critical for Cell Wall Integrity Signaling and Interaction with Host Cells in Cryptococcus neoformans Pathogenesis.

Thak E, Son Y, Lee D, Kim H, Kim J, Lee S mBio. 2022; 13(6):e0211222.

PMID: 36409123 PMC: 9765558. DOI: 10.1128/mbio.02112-22.

Role of Protein Glycosylation in Interactions of Medically Relevant Fungi with the Host.

Gomez-Gaviria M, Vargas-Macias A, Garcia-Carnero L, Martinez-Duncker I, Mora-Montes H J Fungi (Basel). 2021; 7(10).

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Ultrastructural Study of Surface During Budding Events.

S Araujo G, Alcantara C, Rodrigues N, De Souza W, Pontes B, Frases S Front Microbiol. 2021; 12:609244.

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Glycomics, Glycoproteomics, and Glycogenomics: An Inter-Taxa Evolutionary Perspective.

West C, Malzl D, Hykollari A, Wilson I Mol Cell Proteomics. 2020; 20:100024.

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Titan cell production in reshapes the cell wall and capsule composition during infection.

Mukaremera L, Lee K, Wagener J, Wiesner D, Gow N, Nielsen K Cell Surf. 2018; 1:15-24.

PMID: 30123851 PMC: 6095662. DOI: 10.1016/j.tcsw.2017.12.001.

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