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Equilibration of (2)H Labeling Between Body Water and Free Amino Acids: Enabling Studies of Proteome Synthesis

Overview
Journal Anal Biochem
Publisher Elsevier
Specialty Biochemistry
Date 2011 May 21
PMID 21596013
Citations 8
Authors
Affiliations
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Abstract

Protein synthesis can be estimated by measuring the incorporation of a labeled amino acid into a proteolytic peptide. Although prelabeled amino acids are typically administered, recent studies have tested (2)H(2)O; the assumption is that there is rapid equilibration of (2)H (in body water) with the carbon-bound hydrogens of amino acids before those amino acids are incorporated into a protein(s). We have determined the temporal changes in (2)H labeling of body water and amino acids which should build confidence in (2)H(2)O-based studies of protein synthesis when one aims to measure the (2)H labeling of proteolytic peptides.

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