The Human Cytomegalovirus Receptor on Fibroblasts is a 30-kilodalton Membrane Protein

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1990 Jun 1

PMID 2159525

Citations 24

Authors

H P Taylor

N R Cooper

Affiliations

Soon will be listed here.

Abstract

Previous studies have demonstrated that human cytomegalovirus (HCMV) binding to human foreskin fibroblasts (HFF) is mediated by a single type of molecule, likely a glycoprotein, which serves as a specific receptor for the virus. In the present experiments, HCMV was found to bind to an HFF membrane protein with an approximate molecular mass of 30 kilodaltons (kDa); weak binding to 28- and 92-kDa membrane components was also observed. Binding was specific, as it was inhibited by excess unlabeled HCMV. Radiolabeled HCMV also bound selectively to Raji and Daudi lymphoblastoid cell membrane proteins of the same molecular masses. The 30-kDa radiolabeled HFF membrane protein bound to HCMV in solution; this binding was also specific, as it was blocked by an excess of HCMV. These data suggest that a membrane protein with a molecular mass of approximately 30 kDa mediates HCMV binding to several cell types.

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References

Rice G, Schrier R, Oldstone M . Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products. Proc Natl Acad Sci U S A. 1984; 81(19):6134-8. PMC: 391874. DOI: 10.1073/pnas.81.19.6134. View

Stannard L . Beta 2 microglobulin binds to the tegument of cytomegalovirus: an immunogold study. J Gen Virol. 1989; 70 ( Pt 8):2179-84. DOI: 10.1099/0022-1317-70-8-2179. View

Klatzmann D, Champagne E, Chamaret S, Gruest J, Guetard D, Hercend T . T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAV. Nature. 1984; 312(5996):767-8. DOI: 10.1038/312767a0. View

Frade R, Barel M, Klein G . gp140, the C3d receptor of human B lymphocytes, is also the Epstein-Barr virus receptor. Proc Natl Acad Sci U S A. 1985; 82(5):1490-3. PMC: 397288. DOI: 10.1073/pnas.82.5.1490. View

Nemerow G, Wolfert R, McNaughton M, Cooper N . Identification and characterization of the Epstein-Barr virus receptor on human B lymphocytes and its relationship to the C3d complement receptor (CR2). J Virol. 1985; 55(2):347-51. PMC: 254939. DOI: 10.1128/JVI.55.2.347-351.1985. View

Nemerow G, Siaw M, Cooper N . Purification of the Epstein-Barr virus/C3d complement receptor of human B lymphocytes: antigenic and functional properties of the purified protein. J Virol. 1986; 58(2):709-12. PMC: 252969. DOI: 10.1128/JVI.58.2.709-712.1986. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

WENTWORTH B, French L . Plaque assay of cytomegalovirus strains of human origin. Proc Soc Exp Biol Med. 1970; 135(2):253-8. DOI: 10.3181/00379727-135-35031. View

Fiala M, Payne J, Berne T, Moore T, Henle W, Montgomerie J . Epidemiology of cytomegalovirus infection after transplantation and immunosuppression. J Infect Dis. 1975; 132(4):421-33. DOI: 10.1093/infdis/132.4.421. View

10.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

11.

Stinski M . Human cytomegalovirus: glycoproteins associated with virions and dense bodies. J Virol. 1976; 19(2):594-609. PMC: 354895. DOI: 10.1128/JVI.19.2.594-609.1976. View

12.

Elder J, Pickett 2nd R, Hampton J, Lerner R . Radioiodination of proteins in single polyacrylamide gel slices. Tryptic peptide analysis of all the major members of complex multicomponent systems using microgram quantities of total protein. J Biol Chem. 1977; 252(18):6510-5. View

13.

Geelen J, WALIG C, Wertheim P, van der Noordaa J . Human cytomegalovirus DNA. I. Molecular weight and infectivity. J Virol. 1978; 26(3):813-6. PMC: 525906. DOI: 10.1128/JVI.26.3.813-816.1978. View

14.

Keay S, Merigan T, Rasmussen L . Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus. Proc Natl Acad Sci U S A. 1989; 86(24):10100-3. PMC: 298652. DOI: 10.1073/pnas.86.24.10100. View

15.

Nemerow G, Mullen 3rd J, Dickson P, Cooper N . Soluble recombinant CR2 (CD21) inhibits Epstein-Barr virus infection. J Virol. 1990; 64(3):1348-52. PMC: 249254. DOI: 10.1128/JVI.64.3.1348-1352.1990. View

16.

Rowe W, Hartley J, Waterman S, Turner H, HUEBNER R . Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proc Soc Exp Biol Med. 1956; 92(2):418-24. View

17.

Tragardh L, Curman B, Wiman K, Rask L, Peterson P . Chemical, physical-chemical, and immunological properties of papain-solubilized human transplatation antigens. Biochemistry. 1979; 18(11):2218-26. DOI: 10.1021/bi00578a013. View

18.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

19.

Sege K, Rask L, Peterson P . Role of beta2-microglobulin in the intracellular processing of HLA antigens. Biochemistry. 1981; 20(16):4523-30. DOI: 10.1021/bi00519a003. View

20.

Nemerow G, JENSEN F, Cooper N . Neutralization of Epstein-Barr virus by nonimmune human serum. Role of cross-reacting antibody to herpes simplex virus and complement. J Clin Invest. 1982; 70(5):1081-91. PMC: 370321. DOI: 10.1172/jci110696. View