New Insights into the Translocation Route of Enrofloxacin and Its Metalloantibiotics

Overview

Journal J Membr Biol

Date 2011 May 18

PMID 21584680

Citations 7

Authors

C Ribeiro

S C Lopes

P Gameiro

Affiliations

Soon will be listed here.

Abstract

Probing drug/lipid interactions at the molecular level represents an important challenge in pharmaceutical research, drug discovery and membrane biophysics. Previous studies showed that enrofloxacin metalloantibiotic has potential as an antimicrobial agent candidate, since it exhibits antimicrobial effect comparable to that of free enrofloxacin but a different translocation route. These differences in uptake mechanism can be paramount in counteracting bacterial resistance. In view of lipids role in bacterial drug uptake, the interaction of these compounds with different Escherichia coli model membranes were studied by fluorescence spectroscopy. Partition coefficients determined showed that lipid/antibiotic interactions were sensitive to liposomes composition and that the metalloantibiotic had a higher partition than free enrofloxacin. These results corroborate the different mechanism of entry proposed and can be rationalized on the basis that an electrostatic interaction between the metalloantibiotic positively charged species, present at physiological pH, and the lipids negatively charged head groups clearly promotes the lipid/antimicrobial association.

Citing Articles

Fluoroquinolone-Transition Metal Complexes: A Strategy to Overcome Bacterial Resistance.

Ferreira M, Gameiro P Microorganisms. 2021; 9(7).

PMID: 34361943 PMC: 8303200. DOI: 10.3390/microorganisms9071506.

Fluoroquinolone Metalloantibiotics to Bypass Antimicrobial Resistance Mechanisms: Decreased Permeation through Porins.

Ferreira M, Sousa C, Gameiro P Membranes (Basel). 2020; 11(1).

PMID: 33375018 PMC: 7822003. DOI: 10.3390/membranes11010003.

Fluoroquinolone Metalloantibiotics: A Promising Approach against Methicillin-Resistant .

Ferreira M, Bessa L, Sousa C, Eaton P, Bongiorno D, Stefani S Int J Environ Res Public Health. 2020; 17(9).

PMID: 32365881 PMC: 7246690. DOI: 10.3390/ijerph17093127.

Solid lipid nanoparticles with enteric coating for improving stability, palatability, and oral bioavailability of enrofloxacin.

Li C, Zhou K, Chen D, Xu W, Tao Y, Pan Y Int J Nanomedicine. 2019; 14:1619-1631.

PMID: 30880969 PMC: 6402439. DOI: 10.2147/IJN.S183479.

Ciprofloxacin metalloantibiotic: an effective antibiotic with an influx route strongly dependent on lipid interaction?.

Ferreira M, Gameiro P J Membr Biol. 2014; 248(1):125-36.

PMID: 25378125 DOI: 10.1007/s00232-014-9749-6.

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