Identification of Arginine Residues in Peptides by 2D-IR Echo Spectroscopy

Overview

Journal J Phys Chem A

Publisher American Chemical Society

Specialty Chemistry

Date 2011 May 5

PMID 21539337

Citations 20

Authors

Ayanjeet Ghosh

Matthew J Tucker

Robin M Hochstrasser

Affiliations

Soon will be listed here.

Abstract

The CN stretching vibrations of the guanidyl group in the arginine dipeptide side chain are examined by two-dimensional infrared spectroscopy. In D(2)O, the spectra display two distinct diagonal peaks. These nearly degenerate modes undergo ultrafast energy transfer. The energy-transfer rate was determined directly from the 2D-IR spectra to be 1/2.1 ps(-1). The cross peaks in 2D-IR arising from the energy transfer provide a definitive identification of arginine in larger proteins. An example of arginine in the transmembrane protein M2, found in influenza viruses, is given.

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