Protein Dynamics by Two-Dimensional Infrared Spectroscopy

Overview

Journal Annu Rev Anal Chem (Palo Alto Calif)

Publisher Annual Reviews

Specialty Chemistry

Date 2021 Jul 27

PMID 34314221

Citations 3

Authors

Goran W Tumbic

Md Yeathad Hossan

Megan C Thielges

Affiliations

Soon will be listed here.

Abstract

Proteins function as ensembles of interconverting structures. The motions span from picosecond bond rotations to millisecond and longer subunit displacements. Characterization of functional dynamics on all spatial and temporal scales remains challenging experimentally. Two-dimensional infrared spectroscopy (2D IR) is maturing as a powerful approach for investigating proteins and their dynamics. We outline the advantages of IR spectroscopy, describe 2D IR and the information it provides, and introduce vibrational groups for protein analysis. We highlight example studies that illustrate the power and versatility of 2D IR for characterizing protein dynamics and conclude with a brief discussion of the outlook for biomolecular 2D IR.

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