Alpha 2.0

» Articles » PMID: 21535268

Ectopic Study of Tissue-engineered Bone Complex with Enamel Matrix Proteins, Bone Marrow Stromal Cells in Porous Calcium Phosphate Cement Scaffolds, in Nude Mice

Overview

Overview

Journal Cell Prolif

Date 2011 May 4

PMID 21535268

Citations 5

Authors

Authors

X J Wang

H Huang

F Yang

L G Xia

W J Zhang

X Q Jiang

F Q Zhang

Affiliations

Affiliations

Soon will be listed here.

Abstract

Objective: This study aimed to investigate the potential of enamel matrix proteins (EMPs) on promoting osteogenic differentiation of porcine bone marrow stromal cells (pBMSCs), as well as new bone formation capabilities, in a tissue-engineered bone complex scaffold of EMPs, pBMSCs and porous calcium phosphate cement (CPC).

Materials And Methods: Effects of EMPs on pBMSCs in vitro was first determined by alkaline phosphatase (ALP) activity, von Kossa staining assay and mRNA expression of ALP, bone sialoprotein (BSP) and osteocalcin (OCN) genes. Next, an ectopic new bone formation test was performed in a nude mouse model with four groups: CPC scaffold alone; CPC scaffold + EMPs; CPC scaffold + pBMSCs; and CPC scaffold + EMPs + pBMSCs, for 2 or 4 weeks.

Results: ALP activity, von Kossa assay and mRNA expressions of ALP, BSP and OCN genes were all significantly higher with 150 μg/ml EMP treatment in vitro. In nude mice, new bone formation was detected only in the CPC scaffold + EMPs + pBMSCs group at 2 weeks. At 4 weeks, in the tissue-engineered construct there was significantly higher bone formation ability than other groups.

Conclusions: EMPs promoted osteogenic differentiation of pBMSCs, and the tissue-engineered complex of EMPs, pBMSCs and CPC scaffold may be a valuable alternative to be used in periodontal bone tissue engineering and regeneration.

Citing Articles

Prostaglandin EP4 Selective Agonist AKDS001 Enhances New Bone Formation by Minimodeling in a Rat Heterotopic Xenograft Model of Human Bone.

Ukon Y, Nishida M, Yamamori N, Takeyama K, Sakamoto K, Takenaka S Front Bioeng Biotechnol. 2022; 10:845716.

PMID: 35372320 PMC: 8968459. DOI: 10.3389/fbioe.2022.845716.

Apoptosis repressor with caspase recruitment domain enhances survival and promotes osteogenic differentiation of human osteoblast cells under Zoledronate treatment.

Hu L, Han J, Yang X, Wang Y, Pan H, Xu L Mol Med Rep. 2016; 14(4):3535-42.

PMID: 27573706 PMC: 5042767. DOI: 10.3892/mmr.2016.5669.

Bone Tissue Engineering with Multilayered Scaffolds-Part I: An Approach for Vascularizing Engineered Constructs In Vivo.

Sathy B, Mony U, Menon D, Baskaran V, Mikos A, Nair S Tissue Eng Part A. 2015; 21(19-20):2480-94.

PMID: 26262757 PMC: 4605360. DOI: 10.1089/ten.TEA.2015.0098.

Stem Cells and Calcium Phosphate Cement Scaffolds for Bone Regeneration.

Wang P, Zhao L, Chen W, Liu X, Weir M, Xu H J Dent Res. 2014; 93(7):618-25.

PMID: 24799422 PMC: 4107550. DOI: 10.1177/0022034514534689.

Enamel matrix derivative inhibits adipocyte differentiation of 3T3-L1 cells via activation of TGF-βRI kinase activity.

Gruber R, Bosshardt D, Miron R, Gemperli A, Buser D, Sculean A PLoS One. 2013; 8(8):e71046.

PMID: 23951076 PMC: 3741362. DOI: 10.1371/journal.pone.0071046.

References

1.

Tu Y, Woolston A, Faggion Jr C . Do bone grafts or barrier membranes provide additional treatment effects for infrabony lesions treated with enamel matrix derivatives? A network meta-analysis of randomized-controlled trials. J Clin Periodontol. 2009; 37(1):59-79. DOI: 10.1111/j.1600-051X.2009.01499.x. View

2.

Hakki S, Berry J, Somerman M . The effect of enamel matrix protein derivative on follicle cells in vitro. J Periodontol. 2001; 72(5):679-87. DOI: 10.1902/jop.2001.72.5.679. View

3.

Bianco P, Robey P . Stem cells in tissue engineering. Nature. 2001; 414(6859):118-21. DOI: 10.1038/35102181. View

4.

van den Dolder J, Vloon A, Jansen J . The effect of Emdogain on the growth and differentiation of rat bone marrow cells. J Periodontal Res. 2006; 41(5):471-6. DOI: 10.1111/j.1600-0765.2006.00894.x. View

5.

Ganss B, Kim R, Sodek J . Bone sialoprotein. Crit Rev Oral Biol Med. 2000; 10(1):79-98. DOI: 10.1177/10454411990100010401. View

6.

Guida L, Annunziata M, Carinci F, Di Feo A, Passaro I, Oliva A . In vitro biologic response of human bone marrow stromal cells to enamel matrix derivative. J Periodontol. 2007; 78(11):2190-6. DOI: 10.1902/jop.2007.070185. View

7.

Hammarstrom L . Enamel matrix, cementum development and regeneration. J Clin Periodontol. 1997; 24(9 Pt 2):658-68. DOI: 10.1111/j.1600-051x.1997.tb00247.x. View

8.

Tremain N, Korkko J, Ibberson D, Kopen G, DiGirolamo C, Phinney D . MicroSAGE analysis of 2,353 expressed genes in a single cell-derived colony of undifferentiated human mesenchymal stem cells reveals mRNAs of multiple cell lineages. Stem Cells. 2001; 19(5):408-18. DOI: 10.1634/stemcells.19-5-408. View

9.

Jiang X, Zhao J, Wang S, Sun X, Zhang X, Chen J . Mandibular repair in rats with premineralized silk scaffolds and BMP-2-modified bMSCs. Biomaterials. 2009; 30(27):4522-32. PMC: 2871698. DOI: 10.1016/j.biomaterials.2009.05.021. View

10.

Pan Z, Jiang P . Assessment of the suitability of a new composite as a bone defect filler in a rabbit model. J Tissue Eng Regen Med. 2008; 2(6):347-53. DOI: 10.1002/term.103. View

11.

Otsuka T, Kasai H, Yamaguchi K, Nishihara T . Enamel matrix derivative promotes osteoclast cell formation by RANKL production in mouse marrow cultures. J Dent. 2005; 33(9):749-55. DOI: 10.1016/j.jdent.2005.02.006. View

12.

Song A, Shu R, Xie Y, Song Z, Li H, Liu X . A study of enamel matrix proteins on differentiation of porcine bone marrow stromal cells into cementoblasts. Cell Prolif. 2007; 40(3):381-96. PMC: 6496304. DOI: 10.1111/j.1365-2184.2007.00441.x. View

13.

Simmer J, Lau E, Hu C, Aoba T, Lacey M, Nelson D . Isolation and characterization of a mouse amelogenin expressed in Escherichia coli. Calcif Tissue Int. 1994; 54(4):312-9. DOI: 10.1007/BF00295956. View

14.

Ripamonti U, Reddi A . Tissue engineering, morphogenesis, and regeneration of the periodontal tissues by bone morphogenetic proteins. Crit Rev Oral Biol Med. 1997; 8(2):154-63. DOI: 10.1177/10454411970080020401. View

15.

Fincham A, Moradian-Oldak J, Simmer J . The structural biology of the developing dental enamel matrix. J Struct Biol. 1999; 126(3):270-99. DOI: 10.1006/jsbi.1999.4130. View

16.

Hasegawa N, Kawaguchi H, Hirachi A, Takeda K, Mizuno N, Nishimura M . Behavior of transplanted bone marrow-derived mesenchymal stem cells in periodontal defects. J Periodontol. 2006; 77(6):1003-7. DOI: 10.1902/jop.2006.050341. View

17.

Takayama T, Suzuki N, Narukawa M, Tokunaga T, Otsuka K, Ito K . Enamel matrix derivative stimulates core binding factor alpha1/Runt-related transcription factor-2 expression via activation of Smad1 in C2C12 cells. J Periodontol. 2005; 76(2):244-9. DOI: 10.1902/jop.2005.76.2.244. View

18.

Karageorgiou V, Kaplan D . Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005; 26(27):5474-91. DOI: 10.1016/j.biomaterials.2005.02.002. View

19.

Khairoun I, Magne D, Gauthier O, Bouler J, Aguado E, Daculsi G . In vitro characterization and in vivo properties of a carbonated apatite bone cement. J Biomed Mater Res. 2002; 60(4):633-42. DOI: 10.1002/jbm.10132. View

20.

Sheridan M, Shea L, Peters M, Mooney D . Bioabsorbable polymer scaffolds for tissue engineering capable of sustained growth factor delivery. J Control Release. 2000; 64(1-3):91-102. DOI: 10.1016/s0168-3659(99)00138-8. View