Enamel Matrix Derivative Inhibits Adipocyte Differentiation of 3T3-L1 Cells Via Activation of TGF-βRI Kinase Activity

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Aug 17

PMID 23951076

Citations 8

Authors

Reinhard Gruber

Dieter D Bosshardt

Richard J Miron

Anja C Gemperli

Daniel Buser

Anton Sculean

Affiliations

Soon will be listed here.

Abstract

Enamel matrix derivative (EMD), an extract of fetal porcine enamel, and TGF-β can both suppress adipogenic differentiation. However, there have been no studies that functionally link the role of EMD and TGF-β in vitro. Herein, we examined whether TGF-β signaling contributes to EMD-induced suppression of adipogenic differentiation. Adipogenesis was studied with 3T3-L1 preadipocytes in the presence of SB431542, an inhibitor of TGF-βRI kinase activity. SB431542 reversed the inhibitory effect of EMD on adipogenic differentiation, based on Oil Red O staining and mRNA expression of lipid regulated genes. SB431542 also reduced EMD-stimulated expression of connective tissue growth factor (CTGF), an autocrine inhibitor of adipogenic differentiation. Moreover, short interfering (si)RNAs for CTGF partially reversed the EMD-induced suppression of lipid regulated genes. We conclude that the TGF-βRI - CTGF axis is involved in the anti-adipogenic effects of EMD in vitro.

Citing Articles

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