Characterization of Metallo-beta-lactamase VIM-27, an A57S Mutant of VIM-1 Associated with Klebsiella Pneumoniae ST147

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2011 Apr 27

PMID 21518835

Citations 12

Authors

C C Papagiannitsis

S D Kotsakis

E Petinaki

A C Vatopoulos

E Tzelepi

V Miriagou

L S Tzouvelekis

Affiliations

Soon will be listed here.

Abstract

VIM-27 metallo-β-lactamase, an Ala(57) → Ser variant of VIM-1, was identified in three Klebsiella pneumoniae isolates belonging to sequence type 147. bla(VIM-27) was part of a class 1 integron carried by non-self-transferable plasmids. Kinetic parameters and MIC determinations indicated that VIM-27 hydrolyzed most β-lactams, especially imipenem and cefoxitin, less effectively than VIM-1.

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References

Vatopoulos A . High rates of metallo-beta-lactamase-producing Klebsiella pneumoniae in Greece--a review of the current evidence. Euro Surveill. 2008; 13(4). View

Robin F, Aggoune-Khinache N, Delmas J, Naim M, Bonnet R . Novel VIM metallo-beta-lactamase variant from clinical isolates of Enterobacteriaceae from Algeria. Antimicrob Agents Chemother. 2009; 54(1):466-70. PMC: 2798476. DOI: 10.1128/AAC.00017-09. View

Levesque C, Piche L, Larose C, Roy P . PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob Agents Chemother. 1995; 39(1):185-91. PMC: 162507. DOI: 10.1128/AAC.39.1.185. View

Diancourt L, Passet V, Verhoef J, Grimont P, Brisse S . Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. J Clin Microbiol. 2005; 43(8):4178-82. PMC: 1233940. DOI: 10.1128/JCM.43.8.4178-4182.2005. View

Miriagou V, Tzelepi E, Gianneli D, Tzouvelekis L . Escherichia coli with a self-transferable, multiresistant plasmid coding for metallo-beta-lactamase VIM-1. Antimicrob Agents Chemother. 2002; 47(1):395-7. PMC: 149029. DOI: 10.1128/AAC.47.1.395-397.2003. View

Rodriguez-Martinez J, Nordmann P, Fortineau N, Poirel L . VIM-19, a metallo-beta-lactamase with increased carbapenemase activity from Escherichia coli and Klebsiella pneumoniae. Antimicrob Agents Chemother. 2009; 54(1):471-6. PMC: 2798500. DOI: 10.1128/AAC.00458-09. View

Pournaras S, Tsakris A, Maniati M, Tzouvelekis L, Maniatis A . Novel variant (bla(VIM-4)) of the metallo-beta-lactamase gene bla(VIM-1) in a clinical strain of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2002; 46(12):4026-8. PMC: 132756. DOI: 10.1128/AAC.46.12.4026-4028.2002. View

Garau G, Garcia-Saez I, Bebrone C, Anne C, Mercuri P, Galleni M . Update of the standard numbering scheme for class B beta-lactamases. Antimicrob Agents Chemother. 2004; 48(7):2347-9. PMC: 434215. DOI: 10.1128/AAC.48.7.2347-2349.2004. View

Lauretti L, Riccio M, Mazzariol A, Cornaglia G, Amicosante G, Fontana R . Cloning and characterization of blaVIM, a new integron-borne metallo-beta-lactamase gene from a Pseudomonas aeruginosa clinical isolate. Antimicrob Agents Chemother. 1999; 43(7):1584-90. PMC: 89328. DOI: 10.1128/AAC.43.7.1584. View

10.

Docquier J, Lamotte-Brasseur J, Galleni M, Amicosante G, Frere J, Rossolini G . On functional and structural heterogeneity of VIM-type metallo-beta-lactamases. J Antimicrob Chemother. 2003; 51(2):257-66. DOI: 10.1093/jac/dkg067. View

11.

Mavroidi A, Tzelepi E, Tsakris A, Miriagou V, Sofianou D, Tzouvelekis L . An integron-associated beta-lactamase (IBC-2) from Pseudomonas aeruginosa is a variant of the extended-spectrum beta-lactamase IBC-1. J Antimicrob Chemother. 2001; 48(5):627-30. DOI: 10.1093/jac/48.5.627. View

12.

Vatopoulos A, Philippon A, Tzouvelekis L, Komninou Z, Legakis N . Prevalence of a transferable SHV-5 type beta-lactamase in clinical isolates of Klebsiella pneumoniae and Escherichia coli in Greece. J Antimicrob Chemother. 1990; 26(5):635-48. DOI: 10.1093/jac/26.5.635. View

13.

Kotsakis S, Miriagou V, Tzelepi E, Tzouvelekis L . Comparative biochemical and computational study of the role of naturally occurring mutations at Ambler positions 104 and 170 in GES β-lactamases. Antimicrob Agents Chemother. 2010; 54(11):4864-71. PMC: 2976113. DOI: 10.1128/AAC.00771-10. View

14.

Lassaux P, Traore D, Loisel E, Favier A, Docquier J, Sohier J . Biochemical and structural characterization of the subclass B1 metallo-β-lactamase VIM-4. Antimicrob Agents Chemother. 2010; 55(3):1248-55. PMC: 3067066. DOI: 10.1128/AAC.01486-09. View

15.

Gacar G, Midilli K, Kolayli F, Ergen K, Gundes S, Hosoglu S . Genetic and enzymatic properties of metallo-beta-lactamase VIM-5 from a clinical isolate of Enterobacter cloacae. Antimicrob Agents Chemother. 2005; 49(10):4400-3. PMC: 1251544. DOI: 10.1128/AAC.49.10.4400-4403.2005. View

16.

Cornaglia G, Akova M, Amicosante G, Canton R, Cauda R, Docquier J . Metallo-beta-lactamases as emerging resistance determinants in Gram-negative pathogens: open issues. Int J Antimicrob Agents. 2007; 29(4):380-8. DOI: 10.1016/j.ijantimicag.2006.10.008. View