SUMO Modification of Cell Surface Kv2.1 Potassium Channels Regulates the Activity of Rat Hippocampal Neurons

Overview

Journal J Gen Physiol

Specialty Physiology

Date 2011 Apr 27

PMID 21518833

Citations 73

Authors

Leigh D Plant

Evan J Dowdell

Irina S Dementieva

Jeremy D Marks

Steve A N Goldstein

Affiliations

Soon will be listed here.

Abstract

Voltage-gated Kv2.1 potassium channels are important in the brain for determining activity-dependent excitability. Small ubiquitin-like modifier proteins (SUMOs) regulate function through reversible, enzyme-mediated conjugation to target lysine(s). Here, sumoylation of Kv2.1 in hippocampal neurons is shown to regulate firing by shifting the half-maximal activation voltage (V(1/2)) of channels up to 35 mV. Native SUMO and Kv2.1 are shown to interact within and outside channel clusters at the neuronal surface. Studies of single, heterologously expressed Kv2.1 channels show that only K470 is sumoylated. The channels have four subunits, but no more than two non-adjacent subunits carry SUMO concurrently. SUMO on one site shifts V(1/2) by 15 mV, whereas sumoylation of two sites produces a full response. Thus, the SUMO pathway regulates neuronal excitability via Kv2.1 in a direct and graded manner.

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