Conserved Arabidopsis ECHIDNA Protein Mediates Trans-Golgi-network Trafficking and Cell Elongation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2011 Apr 23

PMID 21512130

Citations 66

Authors

Delphine Gendre

Jaesung Oh

Yohann Boutte

Jacob G Best

Lacey Samuels

Robert Nilsson

Tomohiro Uemura

Alan Marchant

Malcolm J Bennett

Markus Grebe

Rishikesh P Bhalerao

Affiliations

Soon will be listed here.

Abstract

Multiple steps of plant growth and development rely on rapid cell elongation during which secretory and endocytic trafficking via the trans-Golgi network (TGN) plays a central role. Here, we identify the ECHIDNA (ECH) protein from Arabidopsis thaliana as a TGN-localized component crucial for TGN function. ECH partially complements loss of budding yeast TVP23 function and a Populus ECH complements the Arabidopsis ech mutant, suggesting functional conservation of the genes. Compared with wild-type, the Arabidopsis ech mutant exhibits severely perturbed cell elongation as well as defects in TGN structure and function, manifested by the reduced association between Golgi bodies and TGN as well as mislocalization of several TGN-localized proteins including vacuolar H(+)-ATPase subunit a1 (VHA-a1). Strikingly, ech is defective in secretory trafficking, whereas endocytosis appears unaffected in the mutant. Some aspects of the ech mutant phenotype can be phenocopied by treatment with a specific inhibitor of vacuolar H(+)-ATPases, concanamycin A, indicating that mislocalization of VHA-a1 may account for part of the defects in ech. Hence, ECH is an evolutionarily conserved component of the TGN with a central role in TGN structure and function.

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