Single-shot Full-field Reflection Phase Microscopy

Overview

Journal Opt Express

Specialties Biophysics
Ophthalmology

Date 2011 Apr 20

PMID 21503067

Citations 14

Authors

Zahid Yaqoob

Toyohiko Yamauchi

Wonshik Choi

Dan Fu

Ramachandra R Dasari

Michael S Feld

Affiliations

Soon will be listed here.

Abstract

We present a full-field reflection phase microscope that combines low-coherence interferometry and off-axis digital holographic microscopy (DHM). The reflection-based DHM provides highly sensitive and a single-shot imaging of cellular dynamics while the use of low coherence source provides a depth-selective measurement. The setup uniquely uses a diffraction grating in the reference arm to generate an interference image of uniform contrast over the entire field-of-view albeit low-coherence light source. We have measured the path-length sensitivity of our instrument to be approximately 21 picometers/Hz that makes it suitable for nanometer-scale full-field measurement of membrane dynamics in live cells.

Citing Articles

Quantitative phase imaging techniques for measuring scattering properties of cells and tissues: a review-part II.

Goswami N, Anastasio M, Popescu G J Biomed Opt. 2024; 29(Suppl 2):S22714.

PMID: 39070593 PMC: 11283205. DOI: 10.1117/1.JBO.29.S2.S22714.

Perspective on quantitative phase imaging to improve precision cancer medicine.

Liu Y, Uttam S J Biomed Opt. 2024; 29(Suppl 2):S22705.

PMID: 38584967 PMC: 10996848. DOI: 10.1117/1.JBO.29.S2.S22705.

Speckle-resolved optical coherence tomography for mesoscopic imaging within scattering media.

Cua M, Blochet B, Yang C Biomed Opt Express. 2022; 13(4):2068-2081.

PMID: 35519275 PMC: 9045937. DOI: 10.1364/BOE.448969.

The effect of pupil transmittance on axial resolution of reflection phase microscopy.

Hyeon M, Park K, Yang T, Kong T, Kim B, Choi Y Sci Rep. 2021; 11(1):22774.

PMID: 34815473 PMC: 8610988. DOI: 10.1038/s41598-021-02188-0.

Inverse scattering for reflection intensity phase microscopy.

Matlock A, Sentenac A, Chaumet P, Yi J, Tian L Biomed Opt Express. 2020; 11(2):911-926.

PMID: 32206398 PMC: 7041473. DOI: 10.1364/BOE.380845.

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