High Conservation of Transcription Factor Binding and Evidence for Combinatorial Regulation Across Six Drosophila Species

Overview

Journal Nat Genet

Specialty Genetics

Date 2011 Apr 12

PMID 21478888

Citations 84

Authors

Qiye He

Anais F Bardet

Brianne Patton

Jennifer Purvis

Jeff Johnston

Ariel Paulson

Madelaine Gogol

Alexander Stark

Julia Zeitlinger

Affiliations

Soon will be listed here.

Abstract

The binding of some transcription factors has been shown to diverge substantially between closely related species. Here we show that the binding of the developmental transcription factor Twist is highly conserved across six Drosophila species, revealing strong functional constraints at its enhancers. Conserved binding correlates with sequence motifs for Twist and its partners, permitting the de novo discovery of their combinatorial binding. It also includes over 10,000 low-occupancy sites near the detection limit, which tend to mark enhancers of later developmental stages. These results suggest that developmental enhancers can be highly evolutionarily constrained, presumably because of their complex combinatorial nature.

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