Out with the Old, in with the New? Comparing Methods for Measuring Protein Degradation

Overview

Journal Cell Biol Int

Specialty Cell Biology

Date 2011 Apr 12

PMID 21476986

Citations 37

Authors

Jonathan W Yewdell

Joshua R Lacsina

Martin C Rechsteiner

Christopher V Nicchitta

Affiliations

Soon will be listed here.

Abstract

Protein degradation is a critical factor in controlling cellular protein abundance. Here, we compare classical methods for determining protein degradation rates to a novel GFP (green fluorescent protein) fusion protein based method that assesses the intrinsic stability of cloned cDNA library products by flow cytometry [Yen et al. (2008) Science 322, 918]. While no method is perfect, we conclude that chimeric gene reporter approaches, though powerful, should be applied cautiously, due principally to GFP (or other reporter tag) interference with protein organelle targeting or incorporation into macromolecular assemblies, both of which cause spuriously high degradation rates.

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