Apoptotic Genes Are Differentially Expressed in Aged Gingival Tissue

Overview

Journal J Dent Res

Specialty Dentistry

Date 2011 Apr 8

PMID 21471327

Citations 37

Authors

O A Gonzalez

A J Stromberg

P M Huggins

J Gonzalez-Martinez

M J Novak

J L Ebersole

Affiliations

Soon will be listed here.

Abstract

Cellular and molecular changes of the periodontium associated with a higher prevalence of oral diseases (e.g., chronic periodontitis) in aged populations have received little attention. Since impaired apoptosis during aging appears to be related to chronic inflammatory disorders, we hypothesized that the expression of genes associated with apoptotic processes are altered in aged healthy and periodontitis-affected gingival tissue. Ontology analysis of 88 genes related to apoptotic pathways was performed in gingival biopsies of healthy and periodontitis sites from young, adult, and aged non-human primates (Macaca mulatta), using the GeneChip® Rhesus Macaque Genome Array. Lower expression of anti-apoptotic and higher expression of pro-apoptotic genes were associated with healthy gingival tissue from young compared with aged animals. Few differences in gene expression were observed in healthy gingival tissue between adult and aged animals. Comparison between healthy and periodontitis gingival tissues showed that the up- or down-regulated apoptotic genes in diseased gingival tissue are different in adults compared with aged animals. These results suggest that apoptotic events normally occurring in gingival tissues could be reduced in aging,and unique aspects of apoptotic pathways are potentially involved in the pathophysiology of periodontal disease in adult vs. aged gingival tissues.

Citing Articles

Single-nucleus transcriptomics uncovers a geroprotective role of YAP in primate gingival aging.

Hu Q, Zhang B, Jing Y, Ma S, Hu L, Li J Protein Cell. 2024; 15(8):612-632.

PMID: 38577810 PMC: 11259548. DOI: 10.1093/procel/pwae017.

DAMPs and alarmin gene expression patterns in aging healthy and diseased mucosal tissues.

Gonzalez O, Kirakodu S, Ebersole J Front Oral Health. 2023; 4:1320083.

PMID: 38098978 PMC: 10720672. DOI: 10.3389/froh.2023.1320083.

Sex and Age Effects on Healthy Gingival Transcriptomic Patterns.

Ebersole J, Kirakodu S, Nguyen L, Gonzalez O J Dent Res. 2023; 102(8):947-956.

PMID: 37232535 PMC: 10399078. DOI: 10.1177/00220345231166310.

Gingival transcriptomic patterns of macrophage polarization during initiation, progression, and resolution of periodontitis.

Gonzalez O, Kirakodu S, Nguyen L, Ebersole J Clin Exp Immunol. 2022; 211(3):248-268.

PMID: 36571202 PMC: 10038328. DOI: 10.1093/cei/uxac122.

Mucosal circadian rhythm pathway genes altered by aging and periodontitis.

Ebersole J, Gonzalez O PLoS One. 2022; 17(12):e0275199.

PMID: 36472983 PMC: 9725147. DOI: 10.1371/journal.pone.0275199.

References

Meka A, Bakthavatchalu V, Sathishkumar S, Lopez M, Verma R, Wallet S . Porphyromonas gingivalis infection-induced tissue and bone transcriptional profiles. Mol Oral Microbiol. 2010; 25(1):61-74. PMC: 3001241. DOI: 10.1111/j.2041-1014.2009.00555.x. View

Fadok V, Bratton D, Konowal A, Freed P, Westcott J, Henson P . Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF. J Clin Invest. 1998; 101(4):890-8. PMC: 508637. DOI: 10.1172/JCI1112. View

Streckfus C, Parsell D, Streckfus J, Pennington W, Johnson R . Relationship between oral alveolar bone loss and aging among African-American and Caucasian individuals. Gerontology. 1999; 45(2):110-4. DOI: 10.1159/000022072. View

Lee H, Chang C, Chi C . Somatic mutations of mitochondrial DNA in aging and cancer progression. Ageing Res Rev. 2010; 9 Suppl 1:S47-58. DOI: 10.1016/j.arr.2010.08.009. View

Back M, Hlawaty H, Labat C, Michel J, Brink C . The oral cavity and age: a site of chronic inflammation?. PLoS One. 2007; 2(12):e1351. PMC: 2131777. DOI: 10.1371/journal.pone.0001351. View

Struillou X, Boutigny H, Soueidan A, Layrolle P . Experimental animal models in periodontology: a review. Open Dent J. 2010; 4:37-47. PMC: 2885595. DOI: 10.2174/1874210601004010037. View

Ebersole J, Steffen M, Gonzalez-Martinez J, Novak M . Effects of age and oral disease on systemic inflammatory and immune parameters in nonhuman primates. Clin Vaccine Immunol. 2008; 15(7):1067-75. PMC: 2446639. DOI: 10.1128/CVI.00258-07. View

Koulouri O, Lappin D, Radvar M, Kinane D . Cell division, synthetic capacity and apoptosis in periodontal lesions analysed by in situ hybridisation and immunohistochemistry. J Clin Periodontol. 1999; 26(8):552-9. DOI: 10.1034/j.1600-051x.1999.260810.x. View

Honda T, Domon H, Okui T, Kajita K, Amanuma R, Yamazaki K . Balance of inflammatory response in stable gingivitis and progressive periodontitis lesions. Clin Exp Immunol. 2006; 144(1):35-40. PMC: 1809628. DOI: 10.1111/j.1365-2249.2006.03028.x. View

10.

Lucas H, Bartold P, Dharmapatni A, Holding C, Haynes D . Inhibition of apoptosis in periodontitis. J Dent Res. 2009; 89(1):29-33. DOI: 10.1177/0022034509350708. View

11.

Huen M, Chen J . The DNA damage response pathways: at the crossroad of protein modifications. Cell Res. 2007; 18(1):8-16. DOI: 10.1038/cr.2007.109. View

12.

Persson R, Lukehart S, Braham P, Oswald T, Persson G . Clinical and microbiological findings in elderly subjects with gingivitis or periodontitis. J Clin Periodontol. 1998; 25(11 Pt 1):897-907. DOI: 10.1111/j.1600-051x.1998.tb02388.x. View

13.

Albandar J, Tinoco E . Global epidemiology of periodontal diseases in children and young persons. Periodontol 2000. 2002; 29:153-76. DOI: 10.1034/j.1600-0757.2002.290108.x. View

14.

Kanehisa M, Goto S, Furumichi M, Tanabe M, Hirakawa M . KEGG for representation and analysis of molecular networks involving diseases and drugs. Nucleic Acids Res. 2009; 38(Database issue):D355-60. PMC: 2808910. DOI: 10.1093/nar/gkp896. View

15.

Nonnenmacher C, Mutters R, de Jacoby L . Microbiological characteristics of subgingival microbiota in adult periodontitis, localized juvenile periodontitis and rapidly progressive periodontitis subjects. Clin Microbiol Infect. 2001; 7(4):213-7. DOI: 10.1046/j.1469-0691.2001.00210.x. View

16.

Holt P, Moss S, Heydari A, Richardson A . Diet restriction increases apoptosis in the gut of aging rats. J Gerontol A Biol Sci Med Sci. 1998; 53(3):B168-72. DOI: 10.1093/gerona/53a.3.b168. View

17.

Marzetti E, Leeuwenburgh C . Skeletal muscle apoptosis, sarcopenia and frailty at old age. Exp Gerontol. 2006; 41(12):1234-8. DOI: 10.1016/j.exger.2006.08.011. View

18.

Lugering A, Lebiedz P, Koch S, Kucharzik T . Apoptosis as a therapeutic tool in IBD?. Ann N Y Acad Sci. 2006; 1072:62-77. DOI: 10.1196/annals.1326.013. View

19.

Gamonal J, Bascones A, Acevedo A, Blanco E, Silva A . Apoptosis in chronic adult periodontitis analyzed by in situ DNA breaks, electron microscopy, and immunohistochemistry. J Periodontol. 2001; 72(4):517-25. DOI: 10.1902/jop.2001.72.4.517. View

20.

Gamonal J, Sanz M, OConnor A, Acevedo A, Suarez I, Sanz A . Delayed neutrophil apoptosis in chronic periodontitis patients. J Clin Periodontol. 2003; 30(7):616-23. DOI: 10.1034/j.1600-051x.2003.00350.x. View